Anatomical, hormonal and molecular caracterization involved in the acquisition of competence and in vitro regeneration of tomato

• Grant number: 13/12209-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2013
• End date: August 31, 2017
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Lázaro Eustaquio Pereira Peres
• Grantee: Marcela Morato Notini
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 15/15177-9 - Spatio-temporal analysis of molecular events involved in tomato in vitro regeneration, BE.EP.DR

Abstract

Adventitious organogenesis is a specific capacity of plants that leads to the in vitro formation of shoot buds and roots in cultured explants. This property has been widely used in biotechnological methods for both micropropagation and genetic engineering. It has been known for long that in vitro regeneration is strongly influenced by numerous factors, including hormone levels. However, little is known about how these hormones act at the cellular and molecular levels, causing changes that lead to the formation of new organs. Recent studies involving signaling and markers have allowed the elucidation of genetic and morphological changes involved mainly in the induction of new organs phase. However, the molecular and structural mechanisms involved in the acquisition of organogenic competence phase still remain obscure. Thus, this study aims at characterizing the structural and genetic changes specifically involved in the acquisition of competence for stems, roots and callus in vitro organogenesis using the tomato cultivar Micro-Tom. This characterization makes it relevant, It is proposed that the understanding of competence acquisition is the key knowledge to allow for the development of less empirical biotechnological protocols in numerous plant species. In addition, the Micro-Tom model itself has been widely used in functional studies involving generation of transgenic plants due to the recent publication of the tomato genome. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZSOGON, AGUSTIN; CERMAK, TOMAS; NAVES, EMMANUEL REZENDE; NOTINI, MARCELA MORATO; EDEL, KAI H.; WEINL, STEFAN; FRESCHI, LUCIANO; VOYTAS, DANIEL F.; KUDLA, JORG; PEREIRA PERES, LAZAR EUSTIQUIO. De novo domestication of wild tomato using genome editing. NATURE BIOTECHNOLOGY, v. 36, n. 12, p. 1211+, DEC 2018. (15/50220-2, 13/18056-2, 13/12209-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NOTINI, Marcela Morato. Understanding hormonal and temporal factors associated with tomato (Solanum lycopersicum L. cv. Micro-Tom) acquisition of competence: key concepts for in vitro shoot regeneration. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.