Evaluation of toxicity of using chemically treated vinasse Oreochromis niloticus (Perciformes: Cichlidae) as test organism

• Grant number: 13/13228-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2013
• End date: July 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Carmem Silvia Fontanetti Christofoletti
• Grantee: Jorge Evangelista Correia
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated research grant: 12/50197-2 - Action of products used in cultivation of sugar cane on non-target organisms, AP.BIOEN.TEM

Abstract

Currently the cane sugar crop is one of the major monocultures of the country, having a strong impact on the economy and also in the environment. The sugar cane vinasse, one of the main waste generated in the processing of sugar cane into ethanol, has a great potential polluter, front large volume produced and their physicochemical characteristics. The pollution of lakes and rivers, by infiltration of waste disposed in the soil as fertilizer, for example, noteworthy since such compounds may contain toxic elements, causing changes in the aquatic and/or terrestrial ecosystems. In order to mitigate these effects, pretreatment of this waste is a viable alternative to reduce impacts into the environment. In this sense, this project provides for the analysis of the toxic potential of sugar cane vinasse chemically treated with lime (CaO) to increase the pH, using fish (Oreochromis niloticus) as a test organism. After adjustment of pH vinasse to a neutral form (pH = 7), individuals will be expose to different sugar cane vinasse dillutions in water. After exposure will be held: a) analysis of their erythrocytes through the micronucleus test with nuclear abnormalities associated and the comet assay, in order to detect possible changes in the nuclear DNA of these animals b) histopathological analyzes of their gills for toxicological analysis in this organ by the direct contact with the water.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORREIA, JORGE E.; CHRISTOFOLETTI, CINTYA AP.; ANSOAR-RODRIGUEZ, YADIRA; GUEDES, THAYS A.; FONTANETTI, CARMEM S.. Comet assay and micronucleus tests on Oreochromis niloticus (Perciforme: Cichlidae) exposed to raw sugarcane vinasse and to phisicochemical treated vinasse by pH adjustment with lime (CaO). Chemosphere, v. 173, p. 494-501, APR 2017. (11/06749-8, 13/13228-0, 12/50197-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CORREIA, Jorge Evangelista. Avaliação da toxicidade da vinhaça tratada quimicamente utilizando Oreochromis niloticus (Perciformes: Cichlidae) como organismo teste. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.