Gene and protein expression of Toll-Like-1, -2, -4 and -6 in neutrophils of normal pregnancies

• Grant number: 13/12624-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2013
• End date: October 31, 2014
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Márcia Guimarães da Silva
• Grantee: Renata Aparecida Camargo Batista
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Toll-like receptors (TLRs) are an important part of the innate immune system, and they are expressed in various human tissues, including gestational tissues. Few studies have focused on the role played by TLRs in pregnancy, and few data are available in relation to the expression of such receptors in maternal neutrophils. Objective: To determine gene and protein expression profile of TLR-1, -2, -4, and -6 in neutrophils throughout normal pregnancy. Patients and Methods: A cross-sectional study will be conducted and it will include 30 pregnant women in each gestational trimester. Samples of 20 mL of peripheral blood will be collected for the isolation of neutrophils by magnetic beads. Messenger RNA from TLR-1,-2,-4, and -6 in maternal neutrophils will be detected by the real-time Polymerase-Chain Reaction (PCR) method and protein detection of TLRs in neutrophils will be evaluated by flow cytometry. Statistical analysis will be performed by respecting the presuppositions determined by the results. The level of significance adopted will be 5%, and the SigmaStat 9.0 (Jandel Corporation) will be used. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MOCO, NATALIA PREARO; CAMARGO BATISTA, RENATA APARECIDA; MARTIN, LAURA FERNANDES; DE OLIVEIRA, LEANDRO GUSTAVO; GARCIA DE LIMA PARADA, CRISTINA MARIA; DIAS-MELICIO, LUCIANE ALARCAO; GOLIM, MARJORIE DE ASSIS; DA SILVA, MARCIA GUIMARAES. Toll-Like Receptor-2 and-4 Expression by Maternal Neutrophils in Preterm Labor. GYNECOLOGIC AND OBSTETRIC INVESTIGATION, v. 83, n. 1, p. 1-8, 2018. (13/12624-9, 11/14195-2)