Several antimicrobial peptides (AMPs) have been isolated from different species that, in general, utilize these small molecules such as defense strategy against a large range of pathogens. Halictine and rondonin are the PAMs selected for this project, which were initially obtained from invertebrate animals and exhibit lytic activity against fungi, bacteria and also against some tumoral cell lines. Therefore, it would be interesting to compare these new peptides, poorly explored, with the others already well-known in the literature. For this, we will use membrane models as synthetic resources capable of mimicking the essential characteristics of a bilayer, and, thereby, analyzing the steps involved in the peptide/membrane interaction. In these studies, it will be employed, in addition to different peptides, distinct membrane compositions, enabling the creation of a more comprehensive scenario that will be monitored by a variety of techniques: ITC (isothermal titration calorimetry), zeta potential, optical microscopy, light scattering, leakage of carboxyfluorescein (CF), fluorescence spectroscopy and CD (circular dichroism). The differences showed for each peptide will be explored, and, in this way, the different techniques chosen may give information about the binding of these peptides to lipid bilayers. All proposed methodologies will be used in a complementary way and analyzed together, seeking, thus, a detailed and complete action mode of these AMPs in membrane models.
News published in Agência FAPESP Newsletter about the scholarship: