Morphological characterization of luteal cells in bitches

• Grant number: 13/22310-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2014
• End date: June 30, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Maria Denise Lopes
• Grantee: Anderson Alves Ribeiro
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The luteal phase of the pregnant and not pregnant female dogs resembles significantly especially in the growth pattern and luteal regression. Specific assessments such as histological and ultrastructural analysis of corpora lutea (CL) may be able to demonstrate differences between cyclic and gestational corpora lutea (CL). The objective of this proposal is to identify the large and small luteal cells within the corpora lutea of bitches in early gestational and cyclical diestrus. Will be used 10 adult females, divided into 2 groups: Group I (cyclical diestrus) - formed by 5 bitches, not inseminated , which will be submitted to ovariohysterectomy (OSH) between day +8 to +9 days of pre-ovulatory surge of LH. Group II (gestational diestrus) - consisting of 5 bitches to be inseminated at D4 and D6 in the days of preovulatory surge of LH, vaginally, with fresh semen. This group will be submitted to ovariohysterectomy between days 8 to 9 of preovulatory surge of LH, 4 to 5 days after the first insemination. The ovaries will be isolated, identified and sectioned longitudinally to count the number of CLs, then the CLs will be withdrawn, isolated and stored for histological processing and ultrastructural analysis through transmission electron microscopy.