Characterization of angiogenic factors present in corpora lutea of pregnant and non-pregnant dogs

• Grant number: 16/15767-3
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): December 01, 2016
• Effective date (End): April 16, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Denise Lopes
• Grantee: Anderson Alves Ribeiro
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 18/00861-0 - Evaluation the mechanism of luteolysis through angiogenic factors present in pregnant and non-pregnant canine corpora lutea, BE.EP.MS

Abstract

The luteal phase in dogs is very similar among pregnant and non-pregnant females. This similarity is one of the most interesting features about the reproduction of canids and although numerous studies have been conducted, the results are still very inaccurate. In this study, the angiogenic factors present in gestational and cyclic corpora lutea (CLs) will be analyzed in order to evaluate the differences between these reproductive phases. To achieve this goal, will be used ovarian samples already collected in the project entitled: "Luteal function in the cyclic and gestational canine diestrous : a cellular approach to genetic and metabolic point of view," FAPESP process: 2011 / 17768-3. From the results obtained by the technique of RNA-Seq, the angiogenic factors will be selected for validation. The monitoring of the estrous cycle and ovariohysterectomy of females in cyclical diestrous (n = 20) and pregnancy (n = 20) were performed and the CLs were collected between days 7-11, 21- 25, 40-44 and 61-64 after pre-ovulatory surge of LH in all females. For analysis of gene profile, CLs of the right ovary were subjected to RNA-Seq technique and subsequently with the results obtained among the angiogenic factors and their receptors, are selected VEGFA, FGF, ANGPT, IGF and its binding proteins (IGFBP ) for validation by qRT-PCR and immunohistochemistry. Expression validation of selected factors will be assessed as normal and variances, it can be used ANOVA or Kruskal Wallis among the group of pregnant and non-pregnant females. The data will be analyzed using the GraphPad Prism program 5. It will be considered the significance level of p <0.05. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RIBEIRO, Anderson Alves. Dynamics of angiogenic factors in corpora lutea of pregnant and pseudopregnant bitches. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.