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Effect of hyperlipidic diets on the variations of glycemia, food intake and hypothalamic AMPK induced by citoglucopenia

Grant number: 13/21016-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2014
Effective date (End): December 31, 2014
Field of knowledge:Biological Sciences - Physiology
Principal Investigator:Eliane Beraldi Ribeiro
Grantee:Brunna Luise Alvarenga e Silva
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Energy homeostasis depends on the perfect interaction between energy intake and expenditure, leading to the stability of body fat stores. Obesity is nowadays a public health issue and the intake of high-fat diets contributes to the obesity epidemic. We have shown that high-fat intake increases body weight and fat content. Nutrients in the diet, such as glucose and fatty acids, reach the central nervous system and interact with hypothalamic neurons that control glycemia, food intake and energy expenditure. The adenosine monophosphate-activate protein kinase (AMPK) acts at the hypthalamus as an energy and glucose sensor, participating in the control of feeding and metabolism of peripheral tissues. In situations of body energy deficiency, as during fasting, AMPK activation stimulates appetite and thus contributes to energy restoration. On the other hand, its activity is decreased after refeeding.It is known that hypothalamic AMPK is modulated by both central and peripheral glucose levels. Central administration os 2-deoxy-glucose (2DG), a non-metabolizable glucose analog, causes cytoglucopenia, leading to responses similar to those elicited by glucose-sensitive neurons, which includes hunger, increased digestive secretions and hyperglycemia.It is not known the effects of neuro-cytoglucopenia on AMPK activity during high-fat intake. The objective of the present project is to evaluate the effect of 2DG administered intracerebroventricularly, in mice consuming diets enriched with n-6 poliunsaturated fatty acids (soy oil) or saturated fatty acids (lard), on hypothalamic AMPK.