Leaf development in Dryopteridaceae species under the morphological approach of Agnes Arber

• Grant number: 13/26191-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2014
• End date: March 31, 2018
• Field of knowledge: Biological Sciences - Botany - Plant Morphology
• Principal Investigator: Gladys Flávia de Albuquerque Melo de Pinna
• Grantee: Rafael da Silva Cruz
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 15/15920-3 - Class I KNOX expression in developing leaves of Mickelia scandens (Dryopteridaceae), BE.EP.DR

Abstract

In recent years, advances in the understanding of leaf development indicate the need of a rescue of Agnes Arber work, which points the leaf as a partial shoot. Ferns record great similarity between leaf development and the development of the whole shoot, and recognized similarities in the apical meristems structure. There is a great organizational versatility of ferns, which, associated with a simpler meristematic structure, can reveal basic principles of plant organogenesis.Dryopteridaceae presents a transition from terrestrial to epiphytic habit, through hemi-epiphyte condition and holds species with simple and compound leaves, passing by a heterophyllous condition. Within the family, there are groups with clear dimorphism between fertile and sterile leaves and species that produce proliferative buds in their leaves, capable of giving rise to a new individual. The great variation in the family makes it a good model for evolutionary study of leaf variation in vascular plants. For this study, we have chosen Elaphoglossum lingua (C. Presl) Brack.; Mickelia scandens (Aubl.) R.C. Moran et al.; Rumohra adiantiformis (G. Forst.) Ching; Megalastrum connexum (Kaulf.) A.R. Sm. & R.C. Moran; Lastreopsis amplissima (C. Presl) Tindale and Polybotrya cylindrica Kaulf. and one species of Lomariopsidaceae: Lomariopsis marginata (Schrad.) Kuh, to be used as external group.Leaves and shoot apexes in different developmental stages will be collected in the field. The material will be subjected to the usual techniques for paraffin embedding, sectioning and staining and preparation for scanning and transmission electronic microscopy. Morphological data will be obtained from observation of individuals in the field, as well as in the collection of the ferns collection at Herbarium SP.Through the study of the growth region (apical meristems and leaf blastozones), we aim to understand how the ontogenetic changes occurred to generate the morphological diversity along the group phylogeny.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CRUZ, RAFAEL; MELO-DE-PINNA, GLADYS F. A.; VASCO, ALEJANDRA; PRADO, JEFFERSON; AMBROSE, BARBARA A.. Class I KNOXIs Related to Determinacy during the Leaf Development of the FernMickelia scandens(Dryopteridaceae). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 12, JUN 2020. (13/26191-7, 15/15158-4, 15/15920-3)

CRUZ, RAFAEL; PRADO, JEFFERSON; DE ALBUQUERQUE MELO-DE-PINNA, GLADYS FLAVIA. Leaf development in some ferns with variable dissection patterns (Dryopteridaceae and Lomariopsidaceae). FLORA, v. 270, SEP 2020. (15/15158-4, 13/26191-7)

CRUZ, RAFAEL; MELO-DE-PINNA, GLADYS F. A.; VASCO, ALEJANDRA; PRADO, JEFFERSON; AMBROSE, BARBARA A.. Class I KNOX Is Related to Determinacy during the Leaf Development of the Fern Mickelia scandens (Dryopteridaceae). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 12, p. 13-pg., 2020-06-01. (15/15920-3, 15/15158-4, 13/26191-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CRUZ, Rafael da Silva. Development of leaves in ferns under the Agnes Arber\'s continuum view of plant morphology. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) São Paulo.