Role of the CHD7 chromatin remodeler protein in human brain tumor cells

• Grant number: 13/23271-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2014
• End date: May 31, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Mari Cleide Sogayar
• Grantee: Raquel Arminda Martinez Machado
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 14/21614-0 - Role of CHD7 chromatin remodeler protein in glioblastoma stem cells (GSCs) maintenance and radioresistance, BE.EP.DR

Abstract

Chromatin remodeler proteins have emerged as critical regulators of chromatin structureplaying a major role during development. The CHD family is the most divergent group ofchromodomain proteins with a unique combination of functional domains. Since thediscovery that CHD7 mutations are the major cause of the CHARGE syndrome inhuman, this gene has been more studied from the functional point of view, however, therole of this enzyme in brain cancer is still to be reported. In this study, we aim toinvestigate the function of CHD7 in human brain tumor cells. Two commonly usedmalignant glioma cell lines, namely: U87 and A172, were selected for CHD7 gain- andloss-of-function experiments in vitro and in vivo. The CHD7 expression pattern in humanglioma patient samples of different malignancy grades will also be characterized. Weexpect our research will greatly contribute to a better understanding of the molecularmechanisms underlying the role of CHD7 chromatin remodeler protein and provideunprecedented insights into cellular pathways and gene networks associated with cancer.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, RAQUEL A. C.; SCHNEIDER, HANNAH; DEOCESANO-PEREIRA, CARLOS; LICHTENSTEIN, FLAVIO; ANDRADE, FERNANDO; FUJITA, ANDRE; TROMBETTA-LIMA, MARINA; WELLER, MICHAEL; BOWMAN-COLIN, CHRISTIAN; SOGAYAR, MARI CLEIDE. CHD7 promotes glioblastoma cell motility and invasiveness through transcriptional modulation of an invasion signature. SCIENTIFIC REPORTS, v. 9, MAR 8 2019. (13/23271-0, 14/21614-0)