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Study of the ETT2 protein secretion system in Enteroaggregative Escherichia coli 042 prototype strain

Grant number: 13/24458-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): March 01, 2014
Effective date (End): February 28, 2017
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Waldir Pereira Elias Junior
Grantee:Roberto Nepomuceno de Souza Lima
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Enteroaggregative Escherichia coli (EAEC) is an emerging pathogen associated with cases of diarrhea in children, immunocompromised patients, and healthy adults passing by endemic areas. This patotype does not present a homogeneous profile regarding the presence of virulence factors found among different serogroups isolates from diarrheal cases, indicating a multifactorial profile for disease development. Analysis of the genome of EAEC strain 042 indicated the presence of genes encoding a type III secretion system (T3SS) which, in other pathogens, is associated with the translocation of effector proteins directly into the cytoplasm of eukaryotic cells, leading to their morphological and functional changes. This system, named ETT2 (Escherichia coli type three secretion system 2), present high similarity to that found in enterohemorrhagic E. coli and the system codified by Salmonella sp. SPI-1 pathogenic island. In this project we aim to study the EAEC 042 ETT2 system, focusing on the protein secretion profile of a mutant deficient on the system, its cellular location and structural components. The results will be important to clarify the pathogenicity of this E. coli pathotype.