To assess the sweat as alternative sample for doping control in sport

• Grant number: 13/25601-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2014
• End date: November 30, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Bruno Spinosa de Martinis
• Grantee: Dayanne Cristiane Mozaner Bordin
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The sport has become a major industry with valuable prizes and great investment. In an attempt to overcome opponents, to improve athletes to illegal means their performance and, with these scientific developments are increasingly sophisticated and innovated. This advancement requires increasingly selective and sensitive analytical methods for the identification of substances that have been used to promote improved performance unlawfully. The use of performance enhancing drugs in sport is banned by the World Anti-Doping Agency (WADA) and a wide range of pharmacological classes of drugs are present on the Prohibited List. The development of new methods for the detection of these substances in biological fluids is important. Conventional biological matrices used for these analyzes are blood, and especially urine, however the use of alternative biological matrices, has received attention in the context of toxicological analyzes. Among these, the sweat that has been used as an alternative for monitoring drug use biological matrix, is a matrix that presents a number of advantages over conventional arrays, including noninvasive collection, larger window of detection, reduced chance of tampering of the sample. The main challenges for the analysis of drugs and / or their metabolites in sweat are: reproducibility, recovery of the analytes and the need for instrumentation with high sensitivity. The objective of this study is to evaluate the possibility of using sweat as an alternative matrix, comparing with conventional matrix, urine, for prohibited substances in sport, doping.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORDIN, DAYANNE MOZANER; BISHOP, DAVID; DE CAMPOS, EDUARDO GERALDO; BLANES, LUCAS; DOBLE, PHILIP; ROUX, CLAUDE; DE MARTINIS, BRUNO SPINOSA. Analysis of Stimulants in Sweat and Urine Using Disposable Pipette Extraction and Gas Chromatography Coupled to Mass Spectrometry in the Context of Doping Control. JOURNAL OF ANALYTICAL TOXICOLOGY, v. 46, n. 9, p. 8-pg., 2021-11-18. (13/25601-7)

BORDIN, DAYANNE MOZANER; BETTIM, BARBARA BELTRAME; PERDONA, GLEICI CASTRO; DE CAMPOS, EDUARDO GERALDO; DE MARTINIS, BRUNO SPINOSA. Understanding alterations on blood and biochemical parameters in athletes that use dietary supplements, steroids and illicit drugs. Toxicology, v. 376, n. SI, p. 75-82, FEB 1 2017. (13/25601-7)

BORDIN, DAYANNE MOZANER; BETTIM, BARBARA BELTRAME; PERDONA, GLEICI CASTRO; DE CAMPOS, EDUARDO GERALDO; DE MARTINIS, BRUNO SPINOSA. Understanding alterations on blood and biochemical parameters in athletes that use dietary supplements, steroids and illicit drugs. Toxicology, v. 376, p. 8-pg., 2017-02-01. (13/25601-7)

PYKE, GRAHAM H.; KALMAN, JOHN R. M.; BORDIN, DAYANNE M.; BLANES, LUCAS; DOBLE, PHILIP A.. Patterns of floral nectar standing crops allow plants to manipulate their pollinators. SCIENTIFIC REPORTS, v. 10, n. 1, FEB 3 2020. (13/25601-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BORDIN, Dayanne Cristiane Mozaner. Sweat as an alternative sample in doping control. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.