Saliva as method diagnostic of the cystic fibrosis

• Grant number: 14/00611-2
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2014
• Effective date (End): July 31, 2017
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal researcher: Carlos Emilio Levy
• Grantee: Aline Cristina Gonçalves
• Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Introduction: cystic fibrosis (CF) is basically characterized by defective ion transport across the cell membrane in epithelial cells of the airways, pancreas, salivary glands, sweat, intestine and reproductive tract, resulting in mucosal secretions too thick and viscous, which cause obstruction at the level of glandular ducts and tubules. Certain systemic diseases, such as CF, may jeopardize the operation of the salivary glands and saliva production, thereby influencing both the amount of saliva produced and the quality of the fluid since it can affect the chemical components and physical properties of the. The reason for the great variability of the effects of CF on the salivary gland is unknown. Many of these studies were undertaken prior to determining the nature of the mutations that correlate with disease severity. The conclusive test for CF is the determination of sweat electrolytes, and this analysis has been used for over 50 years. Are currently accepted universal reference intervals for sweat chlorides, being applicable to all patients, regardless of gender or age. The Sweat Test, iontophoresis is performed by quantitative analysis of sweat ion stimulated by pilocarpine. Objectives: to evaluate the sensitivity, specificity and accuracy of measurement of electrolytes by ion selective electrode technique (equipment used in gas analysis) in saliva for the diagnosis of cystic fibrosis ; compare the chlorine levels and sodium sweat the technique of iontophoresis with the same ions saliva by ion selective electrode technique, both in patients with cystic fibrosis as the group of individuals without the disease, check and compare the levels of chloride and sodium in the saliva with the same ions sweat, using the technique of ion electrode selective (gases), to compare the technique of ion-selective electrode with the titration and flame photometry for analysis of chlorine and sodium in sweat as much saliva. Patients and methods: a case control matched for age. Saliva samples of each individual will be analyzed by the technique of ion-selective electrode and Titrimetry for dosing and metering of chlorine to sodium dosage. The 02 sweat samples from each patient are analyzed by standard techniques of titration (chlorine) and flame photometry (sodium), and the technique of ion-selective electrode. The data will be processed and stored in the database created by the Statistical Package for Social Sciences ® (SPSS) for Windows version 17. The significance level of 5 % of the study will be submitted to descriptive analysis to obtain the absolute correlation of variables analyzed and frequencies. The Mann Whitney test will be used to evaluate the differences between the results of chloride and sodium in saliva (ion selective electrode, for chloride titration and flame photometry for sodium) obtained in patients with CF and individuals without cystic fibrosis, the same will be done with the results of the sweat (ion selective electrode, titration for chloride and flame photometry for sodium), with a significance level of 5 %. The ROC curve is applied to measure the sensitivity, specificity and determine the cut point (cut- off) of chlorine and sodium ions from saliva by selective ion electrode techniques (gas analysis), titration (chloride) and flame photometry (sodium) since to sweat the ROC curve will be applied only to the results of the ion selective electrode technique (gas analysis). Expected results: check the efficiency of the analysis of chlorine and sodium saliva by ion selective electrode technique (blood gas analysis equipment), thus validating the method. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALINE CRISTINA GONÇALVES; FERNANDO AUGUSTO LIMA MARSON; REGINA MARIA HOLANDA MENDONÇA; CARMEN SÍLVIA BERTUZZO; ILMA APARECIDA PASCHOAL; JOSÉ DIRCEU RIBEIRO; ANTÔNIO FERNANDO RIBEIRO; CARLOS EMÍLIO LEVY. Chloride and sodium ion concentrations in saliva and sweat as a method to diagnose cystic fibrosis. Jornal de Pediatria, v. 95, n. 4, p. 443-450, Ago. 2019. Web of Science Citations: 1.

MARIANA ZORRÓN MEI HSIA PU; FLÁVIA CORRÊA CHRISTENSEN-ADAD; ALINE CRISTINA GONÇALVES; WALTER JOSÉ MINICUCCI; JOSÉ DIRCEU RIBEIRO; ANTONIO FERNANDO RIBEIRO. Insulin therapy in patients with cystic fibrosis in the pre-diabetes stage: a systematic review. Revista Paulista de Pediatria, v. 34, n. 3, p. -, Set. 2016.