Effect of addition of calcium and fluoride in hydrogen peroxide bleaching gel on pulp penetration, color and microhardness.

• Grant number: 13/26449-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2014
• End date: March 31, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Carlos Rocha Gomes Torres
• Grantee: Tatiane Josefa da Silva
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The objective of this study will be to evaluate the effects of calcium and fluorideions of addition to the formulation of a 35% hydrogen peroxide bleaching gel in itspenetration through the tooth structure, whitening efficacy and microhardness of dentalenamel. It will be used 80 bovine incisors, from which will be obtained enamel/dentindiscs from the buccal surface, with 6 mm diameter and 2mm thick (1mm of enamel and1mm of dentin). The samples will be divided into four groups depending on theprotective/remineralizing substances added to the hydrogen peroxide gel 35%: GroupCa - 0.5% of Calcium Gluconate; Group F - 0.2% of Sodium Fluoride; Group Ca+F -0.5% Calcium Gluconate and 0.2% Sodium Fluoride; Control Group - no additionalagent will be added. The baseline color and enamel microhardness of the samples willbe measured before bleaching procedures. The specimens of each group will beplaced in a metal holder in which there will be an internal cavity to simulate the pulpchamber, which will be filled with acetate buffer to collect peroxide. The whitening gelswill be applied for 30 min, divided into 3 applications. The amount of peroxide whichpassed through the samples will be determined by absorbance spectrophotometry. Themicrohardness will be measured again immediately after bleaching. The samples willbe immersed in artificial saliva for 7 days, after that the color will be reassessed. Thedata will be statistically analyzed, adopting a significance level of 5%.

Scientific publications

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TORRES, C. R. G.; ZANATTA, R. F.; SILVA, T. J.; BORGES, A. B.. Effect of Calcium and Fluoride Addition to Hydrogen Peroxide Bleaching Gel On Tooth Diffusion, Color, and Microhardness. Operative Dentistry, v. 44, n. 4, p. 424-432, JUL-AUG 2019. (13/26449-4)