Detection of Trypanosoma vivax in bovines experimentally infected using lamp and PCR during apparent aparasitemic phases

• Grant number: 14/04066-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2014
• End date: November 30, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Fabiano Antonio Cadioli
• Grantee: Giuliana Nascimento dos Santos
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study is evaluate loop-mediated isothermal amplification (LAMP) and PCR for molecular diagnostic of Brazilian isolates of Trypanosoma vivax in bovines experimentally infected during apparent aparasitemic phase of infection. T. vivax is a hemoprotozoan that infects equines, bovines, camels and other ruminants, mainly in Africa and South America, where causes relevant economic losses. Reports of outbreaks by T. vivax in ruminant's herds are increasing in the last years in Brazil. Ruminants can be considered T. vivax reservoirs because show low or none parasitemia and lack of clinical signs, which contribute to the parasite spread to non-endemic areas. Thus, faster and precise molecular diagnose techniques are required to stop T. vivax dissemination in Brazilian territory, especially that ones feasible in field conditions like LAMP. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CADIOLI, FABIANO ANTONIO; FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; DOS SANTOS, GIULIANA NASCIMENTO; ANDRE, MARCOS ROGERIO; GARCIA DE ALMEIDA CASTILHO, KAYO JOSE; MACHADO, ROSANGELA ZACARIAS. Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods. Veterinary Parasitology, v. 214, n. 1-2, p. 174-177, NOV 30 2015. (14/04066-9, 12/02284-3, 11/15945-5, 14/11600-1)