Serological and molecular techniques assessing the treatment effectiveness against T. vivax in experimentally infected goats

• Grant number: 14/11600-1
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): September 01, 2014
• Effective date (End): February 28, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Fabiano Antonio Cadioli
• Grantee: Paulo Henrique Sampaio
• Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

T. vivax is responsible by economic losses in livestock worldwide. Infections in ruminant herds have been occurred with increasing frequency in Brazil. However, during the trypanosomiasis course there are fluctuations in parasitemia or aparasitemic intervals, which makes the diagnosis pof this disease hard to do. The "apparently healthy" animals are responsible for the disease introduction in non-endemic areas. Therefore, it becomes essential to use more accurate tools for the diagnosis of this disease such as serological and molecular methods. The aim of this working is use LAMP and PCR molecular tests as tools to confirm parasitological cure of goats experimentally infected by T. vivax following treatment with diaceturate of diaminazeno or hydrochloride of isometamidium. It is expected that the LAMP test will be more sensitive than conventional PCR for detection of T. vivax DNA. The results may contribute to the development of control actions in disease expansion areas. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CADIOLI, FABIANO ANTONIO; FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; DOS SANTOS, GIULIANA NASCIMENTO; ANDRE, MARCOS ROGERIO; GARCIA DE ALMEIDA CASTILHO, KAYO JOSE; MACHADO, ROSANGELA ZACARIAS. Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods. Veterinary Parasitology, v. 214, n. 1-2, p. 174-177, NOV 30 2015. Web of Science Citations: 2.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SAMPAIO, Paulo Henrique. Técnicas sorológicas e moleculares na avaliação da efetividade do tratamento contra Trypanosoma vivax em caprinos experimentalmente infectados. 2017. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias..