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Serological and molecular techniques assessing the treatment effectiveness against T. vivax in experimentally infected goats

Grant number: 14/11600-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2014
Effective date (End): February 28, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Fabiano Antonio Cadioli
Grantee:Paulo Henrique Sampaio
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

T. vivax is responsible by economic losses in livestock worldwide. Infections in ruminant herds have been occurred with increasing frequency in Brazil. However, during the trypanosomiasis course there are fluctuations in parasitemia or aparasitemic intervals, which makes the diagnosis pof this disease hard to do. The "apparently healthy" animals are responsible for the disease introduction in non-endemic areas. Therefore, it becomes essential to use more accurate tools for the diagnosis of this disease such as serological and molecular methods. The aim of this working is use LAMP and PCR molecular tests as tools to confirm parasitological cure of goats experimentally infected by T. vivax following treatment with diaceturate of diaminazeno or hydrochloride of isometamidium. It is expected that the LAMP test will be more sensitive than conventional PCR for detection of T. vivax DNA. The results may contribute to the development of control actions in disease expansion areas. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CADIOLI, FABIANO ANTONIO; FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; DOS SANTOS, GIULIANA NASCIMENTO; ANDRE, MARCOS ROGERIO; GARCIA DE ALMEIDA CASTILHO, KAYO JOSE; MACHADO, ROSANGELA ZACARIAS. Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods. Veterinary Parasitology, v. 214, n. 1-2, p. 174-177, . (12/02284-3, 14/11600-1, 14/04066-9, 11/15945-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SAMPAIO, Paulo Henrique. Serological and molecular techniques in treatment effectiveness evaluation against Trypanosoma vivax in goats experimentally infected. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.