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| Author(s): |
Paulo Henrique Sampaio
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Jaboticabal. 2017-03-10. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2017-02-02 |
| Advisor: | Fabiano Antonio Cadioli |
| Abstract | |
Trypanosoma vivax is a hemoparasite that infects ruminants and causes huge economic losses in livestock, reducing animals productive and reproductive indexes. The main alteration caused by this parasite is anemia and may eventually lead animal to death. The clinical signs are nonspecific which explains the correct diagnosis difficult. Correct diagnosis and treatment are essential for the effective control of this disease. Thus, the goals of this present work was use tools diagnostics molecular (LAMP and PCR) and serological (ELISA and IFAT) to verify the parasitological cure and seronegativation of goats experimentally infected with T. vivax after treatment with diminazene diaceturate (DM) or isometamidium hydrochloride (ISM), besides assess histological alterations in ruminants’ brains to elucidate the mechanism that cause neurologic disorders. In our study we detected anemia and, parasitic resistance to both drugs in animals experimentally infected with T. vivax, which was confirmed by serologic and molecular methods. Additionally, we detected alterations in neuropyl cells of infected animals, which may cause injuries in neuron and consequently neurologic signs in chronic infected animals. Therefore, we conclude that the tested T. vivax isolate was resistant to both drugs, being the best way of diagnosis is the association of serologic and molecular methods and T. vivax cause alterations in neuropyl cells. (AU) | |
| FAPESP's process: | 14/11600-1 - Serological and molecular techniques assessing the treatment effectiveness against T. vivax in experimentally infected goats |
| Grantee: | Paulo Henrique Sampaio |
| Support Opportunities: | Scholarships in Brazil - Doctorate |