Characterization of laccase gene family of eucalyptus (E. grandis)

• Grant number: 14/03241-1
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): May 01, 2014
• Effective date (End): December 31, 2014
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Ivan de Godoy Maia
• Grantee: Mariana de Lara Campos Arcuri
• Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Forestry has shown strong indications of establishment on the national economy due to its intense productivity and also the expansion of industry and the pulp and paper market, both in the country and the world. This scenario is consistent with efforts in the search for biotechnological advances for the industry, which highlights research of obtaining genetically enhanced individuals, with an emphasis on eucalyptus. Laccases, p - diphenol - O2 - oxidoreductase, are enzymes that play a key role in the oxidation of monolignols for lignin biosynthesis, are associated with processes of growth and tolerance to some abiotic stresses, can be found in bacteria, fungi, plants and insects. Studies show that vegetal laccases exhibit similar behavior to those of fungal enzymes, acting to form complementary to lignification in response to oxidative stress route, promoting cellular detoxification. The literature also indicates the correlation of laccases in acclimation of salt stress in corn and tomato. Laccases are usually encoded by multigene families composed of several members. By being involved in lignification processes, the use of laccases for biotechnological processes facing the forest sector can be glimpsed. Assuming the lack of research about the laccases present in the genome of Eucalyptus grandis, an important woody species, this project aims to identify and molecularly characterize this multigene family. The validation of the pattern of tissue - specific expression as well as the verification of the behavior of different members of this family in response to different abiotic stresses will be investigated. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

QUINTERO, FABIOLA OCAMPO; PINTO, LAYRA G.; BARSALOBRES-CAVALLARI, CARLA F.; CAMPOS ARCURI, MARIANA DE LARA; PINO, LILIAN ELLEN; PEREIRA PERES, LAZARO EUSTAQUIO; MALUF, MIRIAN P.; MAIA, IVAN G. Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato. Plant Cell Reports, v. 37, n. 9, p. 1257-1268, SEP 2018. Web of Science Citations: 0.

DE CARVALHO, MARCIO; ACENCIO, MARCIO LUIS; NUNES LAITZ, ALESSANDRA VASCONCELLOS; DE ARAUJO, LAURA MIGLIORINI; CAMPOS ARCURI, MARIANA DE LARA; DO NASCIMENTO, LEANDRO COSTA; MAIA, IVAN G. Impacts of the overexpression of a tomato translationally controlled tumor protein (TCTP) in tobacco revealed by phenotypic and transcriptomic analysis. Plant Cell Reports, v. 36, n. 6, p. 887-900, JUN 2017. Web of Science Citations: 2.