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Analysis of the antiviral action of the curcumin natural compound associated with nanoparticles in HPV-16 positive and negative vulvar carcinoma cell lines

Grant number: 14/02064-9
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): June 01, 2014
Effective date (End): May 31, 2017
Field of knowledge:Biological Sciences - Microbiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Paula Rahal
Grantee:Caroline Measso Do Bonfim
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

Human Papillomavirus (HPV) is a sexually transmitted virus, with more than 170 different types identified. The intraepithelial neoplasia of the vulva (VIN) is a disease associated with HPV, as in 80% of cases, HPV-16 is detected. Currently, there is no effective standard treatment against NIV, and antiviral treatments have adverse effects so that vulvectomy, despite being an invasive method is still widely used. Curcumin is a bioactive natural compound with antioxidant, anti-inflammatory and antineoplastic properties, and appears to have inhibited the expression of HPV oncogenes. However, the low solubility and bioavailability of this compound is a limiting factor. Use of nanoparticles, where curcumin can be encapsulated and delivered into cells at effective concentrations would allow the best performance of this compound in antiviral therapies. Furthermore, nanoparticles as nanospheres and nanoemulsions are considered safe by being biodegradable , and may not be toxic to healthy cells. Thus, the aim of this study is to evaluate the effects of curcumin in carcinoma cell lines vulvar HPV -16 E6 and E7 negative and transducing two distinct HPV -16 variants. Additionally, analysis of the effect of curcumin on the development of organotypic cultures derived from these cells will be performed. The analysis consist of tests for measuring gene expression of oncogenes E5 , E6 and E7 , and immunohistochemical assays for p53 , pRb , caspase 3 and caspase 9 . The results generated in this study are of great importance in the treatment of intraepithelial neoplasia of the vulva and other diseases associated with HPV infection. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DO BONFIM, CAROLINE MEASSO; MONTELEONI, LETICIA FIGUEIREDO; CALMON, MARILIA DE FREITAS; CANDIDO, NATALIA MARIA; SCARIN PROVAZZI, PAOLA JOCELAN; LINO, VANESCA DE SOUZA; RABACHINI, TATIANA; SICHERO, LAURA; VILLA, LUISA LINA; QUINTANA, SILVANA MARIA; DOS SANTOS MELLI, PATRICIA PEREIRA; PRIMO, FERNANDO LUCAS; AMANTINO, CAMILA FERNANDA; TEDESCO, ANTONIO CLAUDIO; BOCCARDO, ENRIQUE; RAHAL, PAULA. Antiviral activity of curcumin-nanoemulsion associated with photodynamic therapy in vulvar cell lines transducing different variants of HPV-16. ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, v. 48, n. 1, p. 515-524, JAN 1 2020. Web of Science Citations: 0.
BADIAL, RODOLFO MIGLIOLI; DIAS, MARINA CARRARA; STUQUI, BRUNA; DOS SANTOS MELLI, PATRICIA PEREIRA; QUINTANA, SILVANA MARIA; DO BONFIM, CAROLINE MEASSO; CORDEIRO, JOSE ANTONIO; RABACHINI, TATIANA; CALMON, MARILIA DE FREITAS; SCARIN PROVAZZI, PAOLA JOCELAN; RAHAL, PAULA. Detection and genotyping of human papillomavirus (HPV) in HIV-infected women and its relationship with HPV/HIV co-infection. MEDICINE, v. 97, n. 14 APR 2018. Web of Science Citations: 3.

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