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Study of apoptosis induced by the Oropouche virus NSs protein

Grant number: 14/02293-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2014
Effective date (End): June 30, 2017
Field of knowledge:Biological Sciences - Microbiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Eurico de Arruda Neto
Grantee:Anibal Silva de Oliveira
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The Oropouche virus (OROV) is a member of Bunyaviridae family, and the second most common cause of arboviral disease in Brazil, but its pathogenesis is largely unknown. OROV causes apoptosis of infected cells, both in vitro and in vivo. Preliminary lab results show that the non-structural protein NSs of OROV induces apoptosis in eukaryotic cells. This project aims to understand the mechanism of action of NSs through biochemical and cell biology approaches. Transfected cells with NSs fused with eGFP will be analyzed by confocal microscopy to determine the subcellular localization of the protein. The involvement of protein pro- and anti-apoptotic Bcl -2 family will be evaluated by pull-down experiments, western blot analysis and quantification of mRNA by qRT-PCR. Targets possibly identified will be confirmed by experiments silencing by RNAi. The recombinant protein NSs will be produced in P. pastoris for the generation of antibodies and for studies of induction of apoptosis by the extrinsic pathway. (AU)

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
OLIVEIRA, Anibal Silva de. Não informado. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto Ribeirão Preto.

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