In vitro antiproliferative activity of Coronarin-D

• Grant number: 14/06636-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2014
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: João Ernesto de Carvalho
• Grantee: Márcia Yumi Okubo
• Host Institution: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA). Universidade Estadual de Campinas (UNICAMP). Paulínia , SP, Brazil

Abstract

In cancer treatment, most of the chemoterapics used are derived from natural products or derivatives. The rhizome of Hedychium coronarium is used in Chinese natural Medicine to treat infections in general, as the sore throat, acute pain related to rheumatism, as a tonic, antipyretic, among other applications. In the literature, there are reports of several biological activities related to its natural compounds. In previous studies, we obtained from the dichloromethane extract of H. coronarium rhizomes the following substances: Coronarin, Coronarin E, Coronarin D, Coronarin cetalizada D, (E)-labda-8(17),12- diene -15,16 - dial and labda -8 (17), 13-dien -15, 16 -olideo. In the assessment of biological activity, Coronarin D showed promisor antiproliferative activity in PC-3 (prostate) and HT-29 (colorectal) tumor cells and was also able to inhibit the phosphatase PTP1B and CDC25B. So, to continue this work, it is proposed to comprove the antiproliferative activity in vitro in cultured tumor cells and evaluate the Coronarin D mechanisms of action, by evaluating the type of cell death, addressing key proteins involved in the apoptotic process, proliferation and survivor. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE PAIVA, PAULA P.; NUNES, JULIA H. B.; NONATO, FABIANA R.; RUIZ, ANA L. T. G.; ZAFRED, RAFAEL R. T.; SOUSA, ILZA M. O.; OKUBO, MARCIA Y.; KAWANO, DANIEL F.; MONTEIRO, PAULA A.; FOGLIO, MARY A.; et al. In Silico, In Vitro, and In Vivo Antitumor and Anti-Inflammatory Evaluation of a Standardized Alkaloid-Enriched Fraction Obtained fromBoehmeria caudataSw. Aerial Parts. Molecules, v. 25, n. 17, SEP 2020. (17/21801-2, 12/19661-4, 11/22458-3, 14/06636-7, 11/22457-7)

FRANCO, YOLLANDA E. M.; OKUBO, MARCIA Y.; TORRE, ADRIANA D.; PAIVA, PAULA P.; ROSA, MARCELA N.; SILVA, VIVIANE A. O.; REIS, RUI M.; RUIZ, ANA L. T. G.; IMAMURA, PAULO M.; DE CARVALHO, JOAO E.; et al. Coronarin D Induces Apoptotic Cell Death and Cell Cycle Arrest in Human Glioblastoma Cell Line. Molecules, v. 24, n. 24, DEC 2019. (16/06137-6, 14/06636-7)

DE PAIVA, PAULA P.; NUNES, JULIA H. B.; NONATO, FABIANA R.; RUIZ, ANA L. T. G.; ZAFRED, RAFAEL R. T.; SOUSA, ILZA M. O.; OKUBO, MARCIA Y.; KAWANO, DANIEL F.; MONTEIRO, PAULA A.; FOGLIO, MARY A.; et al. In Silico, In Vitro, and In Vivo Antitumor and Anti-Inflammatory Evaluation of a Standardized Alkaloid-Enriched Fraction Obtained fromBoehmeria caudataSw. Aerial Parts. Molecules, v. 25, n. 17, p. 20-pg., 2020-09-01. (12/19661-4, 17/21801-2, 11/22457-7, 11/22458-3, 14/06636-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OKUBO, Márcia Yumi. In vitro antiproliferative activity of coronarin D. 2016. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.