In vitro study of anticancer activity of dimeric flavonoid Mplc-2 isolated from vegetable species

• Grant number: 17/01127-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2017
• End date: June 30, 2018
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Giovanna Barbarini Longato
• Grantee: Mayra Cristina Zamana Cubero
• Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil

Abstract

Neoplasm is one of the most deadly diseases in the world. Known as cancer, it is occurs due to multiple genetic mutations in the cells that leads to an abnormal and autonomic proliferation of the cell cycle. The main treatments used for this disease still consist of radiotherapy, surgery and chemotherapy. Some promising chemotherapeutics have been developed in the last decades, among them some drugs originating from natural products and synthetic peptides. Since natural sources are widely found in nature and often their substances are of great therapeutic potential, there is a wide field of studies focusing on natural compounds in the search for new anticancer products. Natural compounds include flavonoids, a broad group of secondary metabolites of polyphenolic plants. Studies developed in recent years have proven that flavonoids are capable of inhibiting cell proliferation, tumor growth and carcinogenesis. It has been widely reported that flavonoids interfere with the initiation, promotion and progression of cancer by modulating different enzymes and receptors responsible for cell proliferation, differentiation, apoptosis, inflammation, angiogenesis, metastasis, and reversal of resistance to multiple drugs. The aim of this project is to evaluate the in vitro anticancer activity of the MPLC-2 flavonoid isolated from the plant species Arrabidaea brachypoda, a plant found in the Brazilian cerrado and that presents medicinal properties as anti-inflammatory, analgesic, antiparasitic and antitumor. Initially, this compound will be evaluated for its potential antiproliferative activity in human tumor cell culture. Subsequently, it will be evaluated for its ability to prevent cell invasion and colony formation. Finally its mechanism of action will be evaluated by the technique of flow cytometry, essential for investigation of the process of cell death. Scientific descriptions of this species still remain insufficient and, therefore, it should be better investigated. The compounds isolated from this species have be enincluded into a rare group of dimeric flavonoids, making the MPLC-2 study even more interesting and favorable. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LIMA, CAROLINA A.; CUBERO, MAYRA C. Z.; FRANCO, YOLLANDA E. M.; RODRIGUES, CARLA D. P.; DO NASCIMENTO, JESSYANE R.; VENDRAMINI-COSTA, DEBORA B.; SCIANI, JULIANA M.; DA ROCHA, CLAUDIA Q.; LONGATO, GIOVANNA B.. Antiproliferative Activity of Two Unusual Dimeric Flavonoids, Brachydin E and Brachydin F, Isolated from Fridericia platyphylla (Cham.) LGLohmann: In Vitro and Molecular Docking Evaluation. BIOMED RESEARCH INTERNATIONAL, v. 2022, p. 12-pg., 2022-02-11. (17/01127-5, 16/06137-6)