Structural differences between two Bothrops neuwiedi SVMPs class P-I

• Grant number: 14/13592-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): March 02, 2015
• Effective date (End): October 01, 2015
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Ana Maria Moura da Silva
• Grantee: Juliana Lech Bernardoni
• Supervisor: R. Manjunatha Kini
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Research place: National University of Singapore (NUS), Singapore
• Associated to the scholarship: 12/23018-0 - Adaptative advantages of the presence of different metalloproteinases in the composition of Bothrops venoms and implications of this variability in snakebites, BP.DR

Abstract

Bothrops venom contains several major components including metalloproteinases, serine proteinases, phospholipase A2 and lectins. Among these components, metalloproteinases (SVMPs) are the most abundant in most Bothrops venoms reaching 38% of the B. neuwiedi pauloensis venom total components. Several conditions such as phylogeny, ontogeny, sex, environment or diet can alter the venom composition, mainly on the SVMPs type and abundance. The mechanism of action of SVMPs is related to the proteolysis of extracellular matrix components, plasma proteins and cell surface proteins. In our Master's project, we identified the presence of several SVMPs in the B. neuwiedi venom. In addition to structural differences, these toxins acted on different targets of mammal hemostatic system. For example, two class P-I have been isolated to homogeneity and their partial amino acid sequences (40% coverage) were identical, although they presented distinct pI and functions. Our goal in this project is to elucidate the complete amino acid sequences of these two SVMPs and their three dimensional structure in order to understand the functional differences between these enzymes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUSA, LEIJIANE F.; PORTES-JUNIOR, JOSE A.; NICOLAU, CAROLINA A.; BERNARDONI, JULIANA L.; NISHIYAMA-, JR., MILTON Y.; AMAZONAS, DIANA R.; FREITAS-DE-SOUSA, LUCIANA A.; MOURAO, ROSA H. V.; CHALKIDIS, HIPOCRATES M.; VALENTE, RICHARD H.; et al. Functional proteomic analyses of Bothrops atrox venom reveals phenotypes associated with habitat variation in the Amazon. JOURNAL OF PROTEOMICS, v. 159, p. 32-46, APR 21 2017. (14/13124-2, 12/16277-9, 14/26058-8, 14/13592-6)