Confirmatory proteomic analysis of a panel of seminal biomarkers of sperm functional abnormalities and semen oxidative stress

• Grant number: 14/11493-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2014
• End date: September 21, 2017
• Field of knowledge: Health Sciences - Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Ricardo Pimenta Bertolla
• Grantee: Paula Intasqui Lopes
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated scholarship(s): 15/18867-6 - Validation of a set of proteins in sperm from fertile and infertile men, BE.EP.DR

Abstract

Semen analysis, routinely utilized during infertility investigation, presents low sensitivity and predictive power, which turns essential the identification of new male infertility diagnostic methods. Taking into consideration that sperm functional abnormalities and semen oxidative stress are considered the main mechanisms associated with infertility, their evaluation can provide additional information regarding male reproductive potential. Accordingly, a previous study from our laboratory performed a proteomic analysis of seminal plasma from normozoospermic men and identified a panel of seminal biomarkers of sperm functional alterations and semen oxidative stress. In order to proceed with this study, the objective of this project is to validate these biomarkers as potential diagnostic methods of alterations in sperm mitochondrial activity, of acrosome defects, of sperm DNA fragmentation, and of high levels of seminal lipid peroxidation. Validation will be performed in three cohorts: (I) validation cohort, with semen samples from our previous study, which were grouped according to sperm functional integrity and seminal levels of lipid peroxidation (n=114), (II) confirmatory prospective cohort, with samples from normozoospermic men without clinical factors of infertility, and (III) confirmatory prospective cohort in different biological conditions (varicocele, obesity, and smoking). For seminal biomarkers evaluation, ELISA or western blot methods will be performed. For the prospective cohorts, sperm functional integrity and seminal levels of lipid peroxidation will also be analyzed. For comparison between the groups, unpaired Student's t- test and multivariate statistical analyses will be performed. With these findings, we expect to find effective seminal biomarkers of sperm functional abnormalities and semen oxidative stress, which may be used during male infertility evaluation. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

INTASQUI, PAULA; BERTOLLA, RICARDO P.; SADI, MARCUS VINICIUS. Prostate cancer proteomics: clinically useful protein biomarkers and future perspectives. EXPERT REVIEW OF PROTEOMICS, v. 15, n. 1, p. 65-79, 2018. (14/11493-0)

CAMARGO, MARIANA; INTASQUI, PAULA; BERTOLLA, RICARDO PIMENTA. Understanding the seminal plasma proteome and its role in male fertility. BASIC AND CLINICAL ANDROLOGY, v. 28, JUN 4 2018. (14/17185-6, 14/11493-0)

INTASQUI, P.; ANTONIASSI, M. P.; CAMARGO, M.; CARVALHO, V.; BERTOLLA, R.. UNDERSTANDING SEMINAL PLASMA PROTEOMIC SHIFTS BROUGHT UPON BY DIVERSE BIOLOGICAL CONDITIONS.. Fertility and Sterility, v. 108, n. 3, p. 2-pg., 2017-09-01. (14/11493-0)

JEREMIAS, JESSICA TIMOTEO; BELARDIN, LARISSA BERLOFFA; OKADA, FATIMA KAZUE; ANTONIASSI, MARIANA P.; FRAIETTA, RENATO; BERTOLLA, RICARDO PIMENTA; INTASQUI, PAULA. Oxidative origin of sperm DNA fragmentation in the adult varicocele. INTERNATIONAL BRAZ J UROL, v. 47, n. 2, p. 275-283, MAR-APR 2021. (19/05879-7, 14/17185-6, 14/11493-0)

CAMARGO, MARIANA; INTASQUI, PAULA; BERTOLLA, RICARDO PIMENTA. Proteomic profile of seminal plasma in adolescents and adults with treated and untreated varicocele. ASIAN JOURNAL OF ANDROLOGY, v. 18, n. 2, p. 194-201, MAR-APR 2016. (14/17185-6, 14/11493-0, 12/15039-7)