Interleukin-1beta, interleukin-6, interleukin-10, tumor necrosis factor-alpha and growth factor transforming-beta1 in keloid

• Grant number: 14/13069-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2014
• End date: January 31, 2015
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Alfredo Gragnani Filho
• Grantee: Olivia Mayumi Tokunaga
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The keloid is the exuberant form of the wound healing process and is developed only in humans, and is more common in tropical countries. Although there are no statistics on this subject, is common in Brazil probably due to the tropical climate and the intense mixing of the population. At all stages of wound healing, growth factors and cytokines play a particularly important role in the interaction with cellular receptors during the inflammatory process. Therefore, Interleukin- 1beta, interleukin-6, interleukin -10, tumor necrosis factor-alpha, and growth factor transforming beta1 cytokines were chosen in order to confirm the expression of the respective genes in keloid tissue samples. The possibility of using the Western blot method in patients with keloids, compared to the control group, in order to build a profile of proteins produced in each group of patients was drawn. This method was chosen for its technical advantages and because the material is directly obtained from the digested tissue.