Effect of titanium tetrafluoride varnish on fibroblasts (NIH3T3): viability assay and cell death

• Grant number: 14/16415-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2014
• End date: September 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Ana Carolina Magalhães
• Grantee: Paula Danielle Rodrigues
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The aim of the project is to evaluate the citoxicity of titanium tetrafluoride (TiF4) compared with sodium fluoride (NaF) varnishes, regardless the fluoride concentration and time of treatment, by analyzing the cell viability and the death type (apoptosis or necrosis). NIH3T3 will be cultured in DMEM supplemented with 10% fetal bovine serum, at 37oC, in a humidified atmosphere of 5% CO2. The cells will be treated with DMEM containing TiF4 or NaF varnishes (0.95%, 1.9% and 2.45% F) in 24-well microplates. After 6, 12 and 24h, the cells will be subjected to viability assay (n=6, MTT) and cell death types will be analyzed (n = 3). The cells will be stained with Hoechst 342 and iodate to differentiate necrosis and apoptosis, as well as they will be examined by the TUNEL method, using fluorescence and confocal microscope, respectively. The data will be subjected to statistical analysis considering the level of significance of 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARANDA SALOMAO, PRISCILA MARIA; DE OLIVEIRA, FLAVIA AMADEU; RODRIGUES, PAULA DANIELLE; AL-AHJ, LUANA POLIONI; DA SILVA GASQUE, KELLEN CRISTINA; JEGGLE, PIA; RABELO BUZALAF, MARILIA AFONSO; DE OLIVEIRA, RODRIGO CARDOSO; EDWARDSON, JOHN MICHAEL; MAGALHAES, ANA CAROLINA. The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time. PLoS One, v. 12, n. 6, JUN 14 2017. (13/20372-0, 14/16415-8, 09/17360-4, 15/05009-1, 10/14984-4, 13/13484-6)