A possible synergistic action of propolis components on the activation of human monocytes

• Grant number: 14/13227-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2014
• End date: July 31, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Ethnopharmacology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: José Maurício Sforcin
• Grantee: Eliza de Oliveira Cardoso
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Propolis has been the subject of several researches due to its biological properties (antibacterial, antiviral, antifungal, anti-inflammatory, immunomodulatory, etc.). Such studies have shown the activities of propolis extracts, not always relating the action of isolated compounds. This project aims to elucidate the biological activities of three phenolic acids found in propolis composition (caffeic acid, dihydrocinnamic acid and p-coumaric acid) collected in the apiary of UNESP. The acids will be combined according to their concentrations in propolis composition, trying to identify a possible synergism similar to propolis action. First, we will evaluate whether combinations have a cytotoxic activity on human monocytes. Then the production of IL-6, IL-10 and TNF-± by monocytes stimulated with LPS, the production of hydrogen peroxide (H2O2), the expression of TLR2, TLR4, HLA-DR and CD80 receptors, and fungicidal activity against Candida albicans will be analyzed. This project will give a better comprehension of propolis action in cells of the immune system and the involvement of its components, leading to a possible development of drugs. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDOSO, ELIZA DE OLIVEIRA; CONTI, BRUNO JOSE; SANTIAGO, KARINA BASSO; CONTE, FERNANDA LOPES; GARCIA OLIVEIRA, LUCAS PIRES; HERNANDES, RODRIGO TAVANELLI; GOLIM, MARJORIE DE ASSIS; SFORCIN, JOSE MAURICIO. Phenolic compounds alone or in combination may be involved in propolis effects on human monocytes. Journal of Pharmacy and Pharmacology, v. 69, n. 1, p. 99-108, JAN 2017. (12/14336-8, 14/13227-6)