Study of alternative ecotoxicological tests in the evaluation of endocrine disruptors in water: emphasis on in vitro assays

• Grant number: 14/22733-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: February 01, 2015
• End date: January 31, 2016
• Field of knowledge: Biological Sciences - Ecology - Applied Ecology
• Principal Investigator: Renata Fracácio Francisco
• Grantee: Juliana Polloni Silva
• Supervisor: José María Navas Antón
• Host Institution: Instituto de Ciência e Tecnologia. Universidade Estadual Paulista (UNESP). Campus de Sorocaba. Sorocaba , SP, Brazil
• Institution abroad: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Spain
• Associated to the scholarship: 12/24495-6 - Study of the behavior of endocrine disrupters in water by chemical analysis and ecotoxicological tests: emphasis on biological recovery, BP.DR

Abstract

Recently, the presence of chemicals with capability to interfere with the function of endocrine systems of animals in their environments has generated a considerable scientific interest, and it has stimulated the development of fast and reliable methods to detect these substances in the wild. The final decision of whether a substance can be classified as an endocrine disruptor is often based on tests in vivo. However, tests in vitro are promising alternatives for these tests, with the advantages of reducing the use of animals, the costs, and the time for obtaining responses. Then, the general objective of this project is to analyse the applicability of tests in vitro to evaluate disruptor present in surface water from Spanish surface waters, and to perform ecotoxicological tests using the estrogenic substances E2 and EE2. Furthermore, we aim to evaluate the descontamination capability after simple adsorption experiments. These data will be compared with results obtained with classical experiments using alive animals developed by us in Brazil, which will permit to seek for a reliable alternative for substituting animals in these analyses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

POLLONI-SILVA, JULIANA; VALDEHITA, ANA; FRACACIO, RENATA; NAVAS, JOSE M.. Remediation efficiency of three treatments on water polluted with endocrine disruptors: Assessment by means of in vitro techniques. Chemosphere, v. 173, p. 267-274, APR 2017. (14/22733-2, 12/14583-5)