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Evaluation of the synthesis and distribution of collagen i, iii, IV, v, VI and its relationship with the end products of advanced glycation in the skin of diabetic rats

Grant number: 14/16496-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2014
Effective date (End): October 31, 2015
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Principal Investigator:Walcy Paganelli Rosolia Teodoro
Grantee:Verônica Protocevich Toledo
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Diabetes mellitus (DM) is a metabolic syndrome characterized by hyperglycemia that resulting in the impaired metabolism of carbohydrates, lipids and proteins. One of the most common complications in these patients is the appearance of skin ulcers associated with peripheral neuropathies. In more severe cases amputation of the affected limb is required, resulting in high surgical costs and recovery of these patients. In more severe cases amputation of the affected limb is required, resulting in high costs for operation and recovery of these patients. It is known that the process of healing of these wounds is different from the physiological process of wound healing in people not affected by diabetes due to dermal accumulation of Advanced Glycation End Products (Advanced Glycation End products - AGEs), which interfere with the synthesis and structure of collagen fibers slowing this process. Previous studies showed that there is decrease in production of type I collagen in the extracellular matrix of the skin tissue of diabetic patients. In this sense, it becomes necessary to study of the organization and distribution of these types of collagens, for better understanding of the healing process in chronic ulcers in diabetic patients. Therefore, since the state of acute hyperglycemia substantially affects the intracellular and extracellular reactions, triggering several chemical and biochemical mechanisms that affect the formation of collagen, we intend to evaluate in this study, the remodeling of collagen types I, III, IV, V and VI in the skin of diabetic rats after 7 and 30 days of induction, in order to try to explain the high fragility and susceptibility to injury in this disease.