Influence of resveratrol in parameters motility and integrity of plasma and acrossomal membranes of boar semen cooled and stored at 15-17°C

• Grant number: 14/19899-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2014
• End date: October 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: André Furugen Cesar de Andrade
• Grantee: Bruno Bracco Donatelli Muro
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This project aims to verify whether the addition of the antioxidant resveratrol generates positive changes in cellular parameters related to the quality of boar semen cooled at 15-17° C. Six ejaculates from six boars (n = 30) will be collected. After semen analyses, the raw semen, will be extended (medium BTS) and added of resveratrol. The following treatments will be performed: Control (CON) BTS without resveratrol, T1 (BTS + RVT 0.1 mM), T2 (BTS + RVT 1.0 mM) and T3 (BTS + RVT 10 mM). Treatments will be cooled and assessed to the characteristics of the motility and integrity of plasma and acrossomal membranes, in the intervals of 0, 24, 48 and 72 hours. The semen analysis will consist in the computerized assessment of the characteristics of motility (CASA) and flow cytometry analysis for the integrity of plasma and acrossomal membranes. . The variables will be submitted to analysis of variance (PROC GLM) and means will be compared by Tukey test.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TORRES, MARIANA ANDRADE; BITTAR RIGO, VICTOR HENRIQUE; LEAL, DIEGO FEITOSA; PINOTI PAVANELI, ANA PAULA; DONATELLI MURO, BRUNO BRACCO; DE AGOSTINI LOSANO, JOAO DIEGO; VECHIATO KAWAI, GIULIA KIYOMI; DEL COLLADO, MAITE; PERECIN, FELIPE; NICHI, MARCILIO; et al. The use of resveratrol decreases liquid-extend boar semen fertility, even in concentrations that do not alter semen quality. Research in Veterinary Science, v. 136, p. 360-368, MAY 2021. (14/19899-6, 14/18573-0)