In vitro evaluation of (anti)mutagenic activity of an aporphine alkaloid and its synthetic intermediates

• Grant number: 14/23950-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2015
• End date: November 09, 2016
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Ana Lúcia Tasca Gois Ruiz
• Grantee: Tamires Carolina Sedano
• Host Institution: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA). Universidade Estadual de Campinas (UNICAMP). Paulínia , SP, Brazil

Abstract

The use of plants as a source of substances with therapeutic action by man dates back centuries and still remains promising currently. Among the various classes of natural products, aporphine alkaloids are widely distributed in plants of the families Annonaceae and Lauraceae among others, showing various pharmacological activities such as dopaminergic, antiproliferative and anti-Leishmania activities in addition to an inhibitory action on cholinesterase, which makes these alkaloids interesting candidates for the treatment of Alzheimer's disease. Several species of Guatteria genus (family Annonaceae) show various oxoaporphine alkaloids with different biological activities and recently the synthesis for aporphine alkaloid, 7-oxo-7H-dibenzo-quinoline-4,5-dimethyl dicarboxylate was described. This alkaloid, together with its three synthetic precursors, showed promising antimicrobial and antiproliferative activities. This project aims to enhance knowledge on efficiency (antimutagenic) and safety of use (mutagenic) of these four compounds through the micronucleus induction assay in CHO-K1 cells (immortalized hamster ovarian cells) and the antioxidant evaluation, through DPPH and Folin-Ciocalteau assays. Key words: cancer, mutagenic activity, micronucleus, antioxidant activity. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, AMANDA A.; FRAJACOMO, SILMARA C. L.; CRUZ, ALLEFE B.; BUGLIO, KAIO EDUARDO; AFFONSO, DANIELE DAIANE; PORTES, MARCELO CECCONI; RUIZ, ANA LUCIA T. G.; DE CARVALHO, JOAO ERNESTO; LUSTRI, WILTON R.; PEREIRA, DOUGLAS H.; et al. Copper(II) and Platinum(II) Naproxenates: Insights on Synthesis, Characterization and Evaluation of Their Antiproliferative Activities. INORGANICS, v. 11, n. 8, p. 21-pg., 2023-08-01. (13/07937-8, 18/12062-4, 21/07458-9, 21/10265-8, 11/01114-4, 14/23950-7, 21/08717-8)

MEZA, EVELENY TIRSA VACA; VASQUEZ-KOOL, JORGE; SANCHEZ, NOE ILDEFONSO COSTILLA; VIEIRA, AMANDIO; RODRIGUES, RODNEY ALEXANDRE FERREIRA; SARTORATTO, ADILSON; GRANADOS, ANGELA DEL PILAR FLORES; TELLO, CARMEN LUISA MARIN; RUIZ, ANA LUCIA TASCA GOIS. Chemical composition and anti-proliferative activity of essential oils from some medicinal plants from Cachicadan, Region La Libertad, Peru. NATURAL PRODUCT RESEARCH, v. N/A, p. 6-pg., 2023-07-15. (14/23950-7, 11/01114-4)

DE OLIVEIRA BRAGA, LUCIA ELAINE; DA SILVA, GISELE GOULART; DE OLIVEIRA SOUSA, ILZA MARIA; SOUZA DE OLIVEIRA, ELLEN CRISTINA; JORGE, MICHELE PEDROZA; MONTEIRO, KARIN MAIA; SEDANO, TAMIRES CAROLINA; FOGLIO, MARY ANN; TASCA GOIS RUIZ, ANA LUCIA. Gastrointestinal effects of Mentha aquatica L. essential oil. INFLAMMOPHARMACOLOGY, v. 30, n. 6, p. 11-pg., 2022-04-22. (11/01114-4, 14/23950-7)

DE AGUIAR, ANA CAROLINA; PEREIRA, GUSTAVO ARAUJO; RIBEIRO, CLAUDIA SILVA DA COSTA; EBERLIN, MARCOS NOGUEIRA; SOARES, LANA PEREIRA; RUIZ, ANA LUCIA TASCA GOIS; PASTORE, GLAUCIA MARIA; MARTINEZ, JULIAN. Capsicum chinense var. BRS Moema: chemical characterization by HPLC-ESI-MS/MS and antiproliferative screening. FOOD & FUNCTION, v. 14, n. 14, p. 11-pg., 2023-06-19. (15/18119-0, 14/23950-7, 17/16903-0, 11/01114-4)