Development of a microsensor coupled to scanning electrochemical microscopy to investigate the role of mitochondria on the respiration process

• Grant number: 14/22396-6
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: January 01, 2015
• End date: December 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Mauro Bertotti
• Grantee: Carla Santana Santos
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 17/12629-1 - Use of multi bipolar electrodes as sensors to monitor cell activity, BE.EP.DD

Abstract

The development of a microelectrode sensor for oxygen monitoring is proposed. The fabricated device will be used as a tip in scanning electrochemical microscopy coupled to a shear force module to oxygen monitoring in vicinity of cellular membrane to investigate the role of mitochondria on the respiration process. Inhibitor species will be used for regulating the function of mitochondrial complexes, enabling the comprehension of the mitochondrial respiration contribution on the consumption of local oxygen. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, CARLA SANTANA; BANNITZ-FERNANDES, RENATA; LIMA, ALEX S.; TAIRUM, CARLOS A.; MALAVAZI, IRAN; NETTO, LUIS E. S.; BERTOTTI, MAURO. Monitoring H2O2 inside Aspergillus fumigatus with an Integrated Microelectrode: The Role of Peroxiredoxin Protein Prx1. Analytical Chemistry, v. 90, n. 4, p. 2587-2593, FEB 20 2018. (13/14955-2, 15/02095-4, 15/20776-9, 14/22396-6)

ALAN R. F. LIMA; ALEX S. LIMA; GABRIEL N. MELONI; CARLA S. SANTOS; MAURO BERTOTTI. Perovskite-Type Oxides La0.6M0.4Ni0.6Cu0.4O3 (M = Ag, Ba, Ce) towards the Oxygen Reduction Reaction (ORR) in Alkaline Medium: Structural Aspects and Electrocatalytic Activity. Journal of the Brazilian Chemical Society, v. 32, n. 3, p. 665-674, 2021-03-01. (14/22396-6, 15/02095-4, 15/20776-9)

SANTOS, CARLA SANTANA; CONZUELO, FELIPE; ESSMANN, VERA; BERTOTTI, MAURO; SCHUHMANN, WOLFGANG. Enhanced sensitivity of scanning bipolar electrochemical microscopy for O-2 detection. Analytica Chimica Acta, v. 1087, p. 36-43, DEC 9 2019. (17/12629-1, 14/22396-6)

ESSMANN, VERA; SANTOS, CARLA SANTANA; TARNEV, TSVETAN; BERTOTTI, MAURO; SCHUHMANN, WOLFGANG. Scanning Bipolar Electrochemical Microscopy. Analytical Chemistry, v. 90, n. 10, p. 6267-6274, MAY 15 2018. (17/12629-1, 14/22396-6)

SANTOS, CARLA SANTANA; KOWALTOWSKI, ALICIA J.; BERTOTTI, MAURO. Single Cell Oxygen Mapping (SCOM) by Scanning Electrochemical Microscopy Uncovers Heterogeneous Intracellular Oxygen Consumption. SCIENTIFIC REPORTS, v. 7, SEP 12 2017. (14/22396-6, 10/51906-1, 13/07937-8)

LIMA, ALEX S.; PRIETO, KATIA R.; SANTOS, CARLA S.; VALERIO, HELLEN PAULA; GARCIA-OCHOA, EVELYN Y.; HUERTA-ROBLES, AURORA; BELTRAN-GARCIA, MIGUEL J.; DI MASCIO, PAOLO; BERTOTTI, MAURO. In-vivo electrochemical monitoring of H2O2 production induced by root-inoculated endophytic bacteria in Agave tequilana leaves. BIOSENSORS & BIOELECTRONICS, v. 99, p. 108-114, JAN 15 2018. (15/02095-4, 14/22396-6, 13/07937-8, 12/12663-1)

SANTOS, CARLA S.; MACEDO, FELIPE; KOWALTOWSKI, ALICIA J.; BERTOTTI, MAURO; UNWIN, PATRICK R.; DA CUNHA, FERNANDA MARQUES; MELONI, GABRIEL N.. Unveiling the contribution of the reproductive system of individual Caenorhabditis elegans on oxygen consumption by single-point scanning electrochemical microscopy measurements. Analytica Chimica Acta, v. 1146, p. 88-97, FEB 15 2021. (13/07937-8, 14/22396-6, 18/08782-1)

LIMA, ALAN R. F.; LIMA, ALEX S.; MELONI, GABRIEL N.; SANTOS, CARLA S.; BERTOTTI, MAURO. Perovskite-Type Oxides La_0.6M_0.4Ni_0.6Cu_0.4O₃ (M = Ag, Ba, Ce) towards the Oxygen Reduction Reaction (ORR) in Alkaline Medium: Structural Aspects and Electrocatalytic Activity. Journal of the Brazilian Chemical Society, v. 32, n. 3, p. 10-pg., 2021-03-01. (15/20776-9, 15/02095-4, 14/22396-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Carla Santana. Development of an electrochemical microsensor for monitoring the respiration activity of single cells and animals by scanning electrochemical microscopy. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.