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Development of a microsensor coupled to scanning electrochemical microscopy to investigate the role of mitochondria on the respiration process

Grant number: 14/22396-6
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): January 01, 2015
Effective date (End): March 13, 2020
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Mauro Bertotti
Grantee:Carla Santana Santos
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):17/12629-1 - Use of multi bipolar electrodes as sensors to monitor cell activity, BE.EP.DD


The development of a microelectrode sensor for oxygen monitoring is proposed. The fabricated device will be used as a tip in scanning electrochemical microscopy coupled to a shear force module to oxygen monitoring in vicinity of cellular membrane to investigate the role of mitochondria on the respiration process. Inhibitor species will be used for regulating the function of mitochondrial complexes, enabling the comprehension of the mitochondrial respiration contribution on the consumption of local oxygen. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANTOS, CARLA SANTANA; CONZUELO, FELIPE; ESSMANN, VERA; BERTOTTI, MAURO; SCHUHMANN, WOLFGANG. Enhanced sensitivity of scanning bipolar electrochemical microscopy for O-2 detection. Analytica Chimica Acta, v. 1087, p. 36-43, DEC 9 2019. Web of Science Citations: 0.
ESSMANN, VERA; SANTOS, CARLA SANTANA; TARNEV, TSVETAN; BERTOTTI, MAURO; SCHUHMANN, WOLFGANG. Scanning Bipolar Electrochemical Microscopy. Analytical Chemistry, v. 90, n. 10, p. 6267-6274, MAY 15 2018. Web of Science Citations: 6.
SANTOS, CARLA SANTANA; BANNITZ-FERNANDES, RENATA; LIMA, ALEX S.; TAIRUM, CARLOS A.; MALAVAZI, IRAN; NETTO, LUIS E. S.; BERTOTTI, MAURO. Monitoring H2O2 inside Aspergillus fumigatus with an Integrated Microelectrode: The Role of Peroxiredoxin Protein Prx1. Analytical Chemistry, v. 90, n. 4, p. 2587-2593, FEB 20 2018. Web of Science Citations: 3.
LIMA, ALEX S.; PRIETO, KATIA R.; SANTOS, CARLA S.; VALERIO, HELLEN PAULA; GARCIA-OCHOA, EVELYN Y.; HUERTA-ROBLES, AURORA; BELTRAN-GARCIA, MIGUEL J.; DI MASCIO, PAOLO; BERTOTTI, MAURO. In-vivo electrochemical monitoring of H2O2 production induced by root-inoculated endophytic bacteria in Agave tequilana leaves. BIOSENSORS & BIOELECTRONICS, v. 99, p. 108-114, JAN 15 2018. Web of Science Citations: 9.
SANTOS, CARLA SANTANA; KOWALTOWSKI, ALICIA J.; BERTOTTI, MAURO. Single Cell Oxygen Mapping (SCOM) by Scanning Electrochemical Microscopy Uncovers Heterogeneous Intracellular Oxygen Consumption. SCIENTIFIC REPORTS, v. 7, SEP 12 2017. Web of Science Citations: 7.

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