Histological evaluation in kidneys of rats treated with different experimental times by fluoride

• Grant number: 14/25167-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2015
• End date: January 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Flávia Godoy Iano
• Grantee: Camila do Carmo Sanchez
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Excessive fluoride (F) intake over a long period of time could result in fluorosis, which can cause dental and skeletal manifestations. Metabolic, functional and structural damages caused by chronic fluorosis have been reported in many tissues. The kidneys are the most responsibility by fluoride excretion, around 60% of fluoride is absolved is excretion by urine in adults. However it appears that there is a time of adjustment of fluoride to the body, so the choice of the experimental time it's important as well as the dose to be administered so that they can find alterations compared to exposure to fluoride. Given this, the objective of this study will be to evaluate the effect of fluoride in rat kidneys by histological analysis. Two groups of Wistar rats are used (n=36), were divided into two time periods (20 and 60 days), which will be divided into a control group (water) and experimental groups (15 ppm and 50 ppm fluoride), which will be administered through drinking water. After experimental period, the animals will be euthanized and kidneys will be collected and divided to the following analysis: histomorphological analyzis, and fluoride. The results will analyzed according to appropriate statistical tests (p <0.05).