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Evaluation the presence of Bacillus cereus and of the microbiological quality of creamy requeijão and especialidade láctea normal and light

Grant number: 14/21534-6
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Start date: January 01, 2015
End date: December 31, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Products of Animal Origin Inspection
Principal Investigator:Luiz Augusto do Amaral
Grantee:Jamile de Oliveira Hachiya
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The search for healthier foods and better life quality is increasing dairy product consumption. Cheese is one of the most consumed product but can consist in risk to consumers when it is contaminated with pathogens. Among the diversity of the cheese that exists in Brazil, stands out tradicional and light creamy "requeijão", as the most known representative, as well their similar: "especialidade láctea". The last differs from the first because, while the "requeijão" is based on milk, "especialidade láctea" has vegetable fat as a non-dairy base. The Bacillus cereus is a pathogen identified as a major problem for the dairy industry. This spore-forming microorganism is able to survive to pasteurization, multiply at low temperatures, produce lipases and proteases causing technological problems in dairy products, and causes diarrhea and emetic syndromes in consumers. The fact of fresh and processed cheeses like "requeijão" and "especialidade láctea" do not pass by salting and maturation process, which inhibits the survival of B. cereus, more the slightly acidic pH, enable the development of this microorganism. Because of these cheeses are with high-fat content, may be more predisposed to the emergence of this bacterium than the light products. Psychrotrophic strains of B. cereus have a better ability to germinate and multiply in milk with high-fat content. To know the existence of possible hygienic deficiencies in these products, it is important to evaluate the presence of groups of microorganisms considered indicators of microbiological quality, as Staphylococcus coagulase-positive and thermotolerant coliforms. On this context, the realization of this project is justified because there are no researches that demonstrate the B. cereus occurrence in these products and the microbiological quality of them, which are among the most dairy products consumed in Brazil.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
JAMILE DE OLIVEIRA HACHIYA; GABRIEL AUGUSTO MARQUES ROSSI; HIGOR OLIVEIRA SILVA; RAFAEL AKIRA SATO; ANA MARIA CENTOLA VIDAL; LUIZ AUGUSTO DO AMARAL. Bactérias do grupo Bacillus cereus como contaminantes em queijos fundidos requeijão e especialidade láctea tipo requeijão. Arq. Inst. Biol., v. 85, . (14/21534-6)
HACHIYA, JAMILE DE OLIVEIRA; MARQUES ROSSI, GABRIEL AUGUSTO; RIBEIRO, LARYSSA FREITAS; SATO, RAFAEL AKIRA; SILVA, HIGOR OLIVEIRA; CENTOLA VIDAL, ANA MARIA; DO AMARAL, LUIZ AUGUSTO. Methicillin-resistant Staphylococcus spp. isolated from curd cheese "requeijao" and "especialidade lactea type requeijao" sold in Brazil. Ciência Rural, v. 47, n. 7, p. 6-pg., . (14/21534-6)
HACHIYA, JAMILE DE OLIVEIRA; MARQUES ROSSI, GABRIEL AUGUSTO; RIBEIRO, LARYSSA FREITAS; SATO, RAFAEL AKIRA; SILVA, HIGOR OLIVEIRA; CENTOLA VIDAL, ANA MARIA; DO AMARAL, LUIZ AUGUSTO. Methicillin-resistant Staphylococcus spp. isolated from curd cheese ``requeijao{''} and ``especialidade lactea type requeijao{''} sold in Brazil. Ciência Rural, v. 47, n. 7, . (14/21534-6)