| Grant number: | 14/20360-4 |
| Support Opportunities: | Scholarships in Brazil - Master |
| Start date: | March 01, 2015 |
| End date: | June 12, 2016 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Reproduction |
| Agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Maria Eugênia Zerlotti Mercadante |
| Grantee: | Erika Aline Ribeiro Dias |
| Host Institution: | Instituto de Zootecnia. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Nova Odessa , SP, Brazil |
Abstract One of the main steps in the cryopreservation of semen is the cooling system. The rate of cooling can reduce the heat shock to the sperm and damages, and thereby the cryopreservation better results can be obtained using slower and more homogeneous cooling. However, in some refrigeration systems, such as domestic refrigerators, it does not. The objective is to confront simultaneously five cooling systems and two cryopreservation of bovine semen, through its effects on the quality of cryopreserved semen. The experimental design will be randomized in a 5x2 factorial arrangement with 5 cooling systems and two cryopreservation, totaling 10 treatments. Two will be used ejaculates from 15 Nelore bulls totaling 30 ejaculates being collected by electroejaculation. The ejaculates are diluted in a single fraction (BotuBov®) to final concentration of 60x106 spz/ml. After dilution of semen, will be cooled for 5 hours in 5 cooling systems (TK 4000® with cooling 0,25ºC/minute curve; TK 4000® with cooling of 0.5°C/min curve, refrigerator Minitüb® 518C, BotuTainer® and common refrigerator) and two cryopreservation (TK 4000® and Styrofoam with liquid nitrogen). Sperm kinetics will be performed by the device IVOS (version 14, Hamilton-Thorne Bioscience) on three occasions: after 5 hours of cooling (before cryopreservation), after cryopreservation and after the Rapid Test Thermotolerance (RTT). Furthermore, the assessment of the acrosome integrity of mitochondrial and plasma membrane is carried out using combination of fluorescent probes. The analyzes of the data generated will help elucidate which better system cooling and cryopreservation that may benefit the technicians who work in the field. (AU) | |
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