Viability analysis of murine experimental model for Prostate Cancer through orthotopic injection of carcinogen

• Grant number: 14/23432-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2015
• End date: January 31, 2016
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Alberto Azoubel Antunes
• Grantee: Felipe Seabra Fernandes
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The Prostate Cancer (PCa) is the most frequent cancer and second leading cause of cancer death in men, as well as being responsible for high costs to society. Due to the high social and economic impact of this pathology, a complete understanding of the process of carcinogenesis and natural history of the disease would enable the definition of the best time for treatment. Thus, the development of experimental models for the PCa, as well as their characterization, is a critical step in the study of this disease. There are several experimental models (xenografts of PC3 cell lines, LNCaP, TRAMP models, Pten gene Knock-Out models, chemical agents cancer induction models such as with DMAB+ testosterone, or N-N-metill nitrosurea + testosterone) that would help in this definition, but all have restrictions. It would be desirable to have a model in which the origin of the malignant lesions were exclusively from the dorsolateral prostate, the portion having the most homology to human prostate peripheral zone, where the majority of tumors arise. Finally, experimental models in PCa assist in understanding the pathogenesis and natural history of the disease, in defining the best time and the most appropriate form of treatment, and in forming the basis for new in vivo prophylactic strategy tests and new drug therapies that could not be tested in humans. In this picture, the project's purpose is to establish an orthotopic experimental model for PCa in Fisher 344 rats and to evaluate its viability.