Carbohydrate restriction effect on AOX flies development

• Grant number: 15/02174-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2015
• End date: February 28, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Marcos Túlio de Oliveira
• Grantee: Marina Minari Chioda
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 14/02253-6 - Investigating the metabolic alterations caused by the transgenic expression of the mitochondrial alternative oxidase of Ciona intestinalis in Drosophila melanogaster, AP.JP

Abstract

In theory, the activity of the mitochondrial alternative oxidase (AOX) decreases ATP production by the respiratory chain of fruitflies that express this enzyme transgenically. Preliminary data show that carbohydrate poor diets have a significant impact on the development of AOX-expressing flies. To analyze in details this negative effect of AOX in conditions of nutritional stress, diverse diets will be tested, in comparison to the standard laboratory diet (rich in sugars, such as sucrose, glucose, corn flour, soy flour and treacle). The carbohydrate-restricted diets will contain only 0, 5 or 10% glucose, in addition to 3.5% yeast extract (source of amino acids and some fatty acids). Optimization of methods for analyzing the mitochondrial proteomics will be performed to provide a starting point to the investigation into the molecular nature of the developmental defect caused by AOX.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAARI, SINA; GARCIA, GEOVANA S.; BREMER, KATHARINA; CHIODA, MARINA M.; ANDJELKOVIC, ANA; DEBES, PAUL V.; NIKINMAA, MIKKO; SZIBOR, MARTEN; DUFOUR, ERIC; RUSTIN, PIERRE; et al. Alternative respiratory chain enzymes: Therapeutic potential and possible pitfalls. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, v. 1865, n. 4, p. 13-pg., 2019-04-01. (14/02253-6, 16/00013-3, 15/02174-1)

SAARI, SINA; GARCIA, GEOVANA S.; BREMER, KATHARINA; CHIODA, MARINA M.; ANDJELKOVIC, ANA; DEBES, PAUL V.; NIKINMAA, MIKKO; SZIBOR, MARTEN; DUFOUR, ERIC; RUSTIN, PIERRE; et al. Alternative respiratory chain enzymes: Therapeutic potential and possible pitfalls. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, v. 1865, n. 4, SI, p. 854-866, APR 1 2019. (15/02174-1, 14/02253-6, 16/00013-3)