Molecular identification of Perlidae (Plecoptera) from Intervales State Park, São Paulo State

• Grant number: 14/25476-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2015
• End date: December 31, 2015
• Field of knowledge: Biological Sciences - Zoology - Taxonomy of Recent Groups
• Principal Investigator: Pitágoras da Conceição Bispo
• Grantee: Lucas Henrique de Almeida
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

In this research plan, specimens Perlidae (Plecoptera) will be collected in Intervales State Park (PEI), State of São Paulo in order to: 1) identify morphologically the species of Perlidae from PEI and to obtain the sequences of the mitochondrial gene COI for each of them; 2) obtain the relationship between adult and nymph using sequences of the mitochondrial gene COI; 3) train students in aquatic entomology specifically on the use of morphological and molecular tools in biodiversity studies. The sampling will be carried out in streams of PEI using D net in the case of nymphs, and nets and light traps for adults. The specimens will be placed in absolute alcohol and stored in a freezer (-20). The species will be identified morphologically based on adult males and subsequently characterized based on the mitochondrial gene COI. Additionally, nymphs will be separated into morphotypes and also be characterized in relation to this gene. Subsequently the sequences are compared in an attempt to associate nymphs and adults. The nymphs associated will be described. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ALMEIDA, LUCAS HENRIQUE; CARDOSO-LEITE, RICARDO; BOA DEODATO, MARIANA FONTE; BISPO, PITAGORAS DA CONCEICAO. Anacroneuria iporanga (Plecoptera: Perlidae): description of the nymph and biological notes. Zootaxa, v. 4550, n. 1, p. 141-145, JAN 23 2019. (14/25476-0, 12/21196-8, 17/16501-0, 15/22008-9)