Molecular and serological detection of Ehrlichia canis in dogs and their respective ticks in two endemic regions

• Grant number: 14/26461-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2015
• End date: April 30, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Katia Denise Saraiva Bresciani
• Grantee: Bruno César Miranda Oliveira
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

This work will be molecularly detect the occurrence of Ehrlichia sp. in the guts, ovary and salivary gland of ticks Rhipicephalus sanguineus, ectoparasites of dogs in two endemic regions for this infection. The organs of ticks, as well as lymph node and bone marrow samples will be tested in dogs using the Polymerase Chain Reaction (PCR) Real-time quantitative for Ehrlichia canis , based on a dsb gene fragment. Dogs blood samples will be analyzed by immunofluorescence Reaction (IFA). Statistical analysis will consist of the chi-square test to assess the association between the presence and absence of this parasite in dogs and their ticks. Also the McNemar test is used to compare between the positive PCR Real-time and IFA, along with the Kappa coefficient to evaluate the concordance between them. Statistical analyzes will be performed with the computer program SAS® (Statistical Analysis System, 2014) and will be considered significant when P <0.05. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIRANDA OLIVEIRA, BRUNO CESAR; FERRARI, ELIS DOMINGOS; VIOL, MILENA ARAUZ; ANDRE, MARCOS ROGERIO; MACHADO, ROSANGELA ZACARIAS; COSTA DE AQUINO, MONALLY CONCEICAO; INACIO, SANDRA VALERIA; GOMES, JANCARLO FERREIRA; GUERRERO, FELIX D.; SARAIVA BRESCIANI, KATIA DENISE. Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues From Rhipicephalus sanguineus (Acari: Ixodidae) Ticks in Areas Endemic for Canine Monocytic Ehrlichiosis in Brazil. Journal of Medical Entomology, v. 56, n. 3, p. 828-831, MAY 2019. (14/26461-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Bruno César Miranda. Molecular detection of Ehrlichia canis in dogs and organs of their respective ticks. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba Araçatuba.