Effects of local administration of Tiludronate on experimental periodontitis in diabetic rats: a microcomputed tomography analysis

• Grant number: 15/02204-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2015
• End date: October 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Flávia Aparecida Chaves Furlaneto
• Grantee: Thais Tedeschi dos Santos
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Therapeutic approaches based on the modulation of the host immunoinflammatory response have been studied and are especially important for patients presenting risk factors for periodontitis, as diabetic patients. The bisphosphonate Tiludronate (TIL) presents anti-resorptive and anti-inflammatory effects. It has not been studied in the association periodontitis-diabetes mellitus (DM) so far. The purpose of this study is to evaluate the effects of local administration of TIL on experimental periodontitis (EP) in rats with and without streptozotocin (STZ)-induced DM. Sixty-four adult male rats (Laboratory Animal Care Facility, Ribeirao Preto School of Dentistry/University of Sao Paulo - FORP/USP) will be used. They will be randomly assigned to one of 8 experimental groups (n = 8): NDM-C, NDM-EP, NDM-EP-TIL 1, NDM-EP-TIL 3, DM-C, DM-EP, DM-EP-TIL 1 and DM-EP-TIL 3 (NDM-animals without DM, C-Control, DM-animals with DM). In the 1st day, DM will be induced in the animals of groups DM through an intraperitoneal injection of STZ. In the 8th day, a ligature will be placed in the cervical area of right mandibular first molars of each rat, except for the rats from groups C. 20 uL TIL solutions in the dosages of 1 mg/kg body weight (for groups NDM-EP-TIL 1 and DM-EP-TIL 1) or 3 mg/kg body weight (for groups NDM-EP-TIL 3 and DM-EP-TIL 3) will be injected into the gingiva in the buccal area adjacent to right mandibular first molars every other day. Animals will be euthanized at the 18th day. Microcomputed tomography analysis will be performed in order to evaluate the alveolar bone. Data will be statistically analyzed.