Effect of physical exercise and high fat diet on mesenteric lymph node lymphocytes in a murine melanoma model

• Grant number: 14/17201-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2015
• End date: December 31, 2016
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Renata Gorjao
• Grantee: Vinicius Leonardo Sousa Diniz
• Host Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil

Abstract

Melanoma is a type of tumor with an incidence of around 4.1% per year (more than 80,000 new cases), that has increased dramatically over the last 50 years. Due to their inherent aggressive nature, melanoma is classified as an important world health problem. The increase in adipose tissue leading to obesity causes changes in immune cell function, generating chronic inflammation that can favor associated diseases development, such as cancer. However, moderate exercise can promote the reestablishment of immune system balance due to the modulation of adipose tissue function. Based on the above observations, the objective of this study is to evaluate the changes in lymphocytes from mesenteric lymph nodes in animals subjected to high-fat diet and moderate exercise in a murine melanoma model. Initially, the animals will be divided into four groups: 1) High fat diet (HL); 2) High fat diet + Moderate exercise (HLE); 3) Normolipidic diet (NL); 4) Normolipidic diet + Moderate exercise (NLE). After eight weeks of treatment of the animals with the diets and treadmill exercise (60% VO2max) performed for 5 days per week, the application of B16F10 melanoma cells will be held. After 21 days, the following parameters will be evaluated: the serum concentration of leptin and adiponectin by ELISA; proliferative capacity of lymphocytes from lymph nodes by BrdU incorporation; lymphocyte viability by Annexin-FITC and propidium iodide incorporation; expression of CD25, CD28 and CD95 on the membrane of effector T lymphocytes and the percentage of regulatory T cells (CD4 +, CD25 + and FoxP3 +) and Th17 by flow cytometry. A better understanding of the mechanisms involved in the modulation of immune response in a of melanoma framework promoted by the development of obesity and changes caused by exercise in a chronic inflammation in model can help the search for new targets for the prevention and treatment of these pathologies.