Assessment of bone repair critical defects treated with biphasic calcium phosphate and low-level laser therapy. Histomorphometric study in rats

• Grant number: 15/09614-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2015
• End date: June 30, 2016
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Andrea Carvalho De Marco
• Grantee: Tatiana Camacho Ribeiro
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

This study aims to investigate the effects of low-level laser therapy associated toBoneCeramic® in critical defects in rats. Forty eight adult rats (Rattus norvegicus, Albinusvariation, Wistar) will be used, with 90 days of age, weighing approximately 300g. Criticaldefects will be made in the calvaria's bone with 5 mm diameter. The animals will berandomly divided into 4 groups: G1-bone defect + blood clot, G2-bone defect + biphasiccalcium phosphate (BCP), G3-bone-defect + LLLT, G4-bone-defect + biphasic calciumphosphate (BCP) + LLLT. Each group will be subdivided in accordance with theobservational periods of 30 to 60 days, with 6 mice in each subgroup. A low-power GaAlAswith a wavelength of 660 nm, 30 mW, 0.04 cm2 area. LLLT will be applied on 5 points sothat the entire wound uniformly receive treatment. Four points of application will bedistributed along the edges of the surgical wound in clockwise from 3, 6, 9 and 12 hours,respectively. And one point in the central region of the surgical wound. Irradiation will bereleased by 12 seconds per point (total time of exposure: 1 minute), with a total energydensity of 45 J/cm2. Laser irradiation will happen immediately after the procedure. On 30 and60 days, 6 animals in each group will be sacrificed by overdose of general anesthesia. Thenthe calvaria will be removed for histological, histomorphometric and analysis. All data willbe statistically analyzed by ANOVA complemented by Tukey test to verify the statisticaldifferences between groups. significance level will be 5%.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

DE MARCO, ANDREA CARVALHO; TORQUATO, LETICIA CAVASSINI; RIBEIRO, TATIANA CAMACHO; NUNES, CAMILLA MORETTO; BERNARDO, DANIELLA VICENSOTTO; MACIEL, CLARISSA CARVALHO MARTINS; PEREIRA, KAUE ALBERTO; JARDINI, MARIA APARECIDA NEVES; SANTAMARIA, MAURO PEDRINE. Effect of Photobiomodulation Therapy Associated With Biphasic Phosphate Calcium on Bone Repair: A Histomorphometric Study in Rats. JOURNAL OF LASERS IN MEDICAL SCIENCES, v. 13, p. 8-pg., 2022-08-15. (15/09614-7)