Molecular detection and characterization of Bartonella spp. in bats sampled in the states of Pará and Paraná, Brazil

• Grant number: 15/04773-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2015
• End date: February 28, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcos Rogério André
• Grantee: Priscila Ikeda
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The genus Bartonella spp. comprises gram-negative and facultative intracellular bacteria which parasitize mainly mammalian erythrocytes and endothelial cells, including those from human beings. Diseases caused by this group of parasites can manifest as self-limiting presentation or as potentially fatal outcomes. These bacteria are the etiological agents of Cat Scratch Disease (CSD), Bacillary Angiomatosis, Trench Fever and other systemic abnormalities in humans, showing their zoonotic potential, especially in immunocompromised individuals. Bartonella species have been showing high prevalence among mammal communities, and bats are the second main reservoir. In front of that, this study aims to determine which species or genotypes of Bartonella spp. circulates in bats population sampled in the states of Pará and Paraná, using molecular techniques. For this purpose, bats spleen and liver DNA samples will be submitted to real-time quantitative PCR (qPCR) for Bartonella spp. based on nuoG gene. Positive samples will be molecularly characterized by conventional PCR assays for Bartonella spp. based on ITS region and rpoB, ribC, gltA, fstZ, pap-31 and groESL genes, followed by sequencing and phylogenetic analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

IKEDA, P.; SEKI, M. C.; CARRASCO, A. O. T.; RUDIAK, L. V.; MIRANDA, J. M. D.; GONCALVES, S. M. M.; HOPPE, E. G. L.; ALBUQUERQUE, A. C. A.; TEIXEIRA, M. M. G.; PASSOS, C. E.; et al. Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil. EPIDEMIOLOGY AND INFECTION, v. 145, n. 10, p. 2038-2052, JUL 2017. (15/14896-1, 15/04773-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

IKEDA, Priscila. Ocurrence and molecular characterization of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled in Brazil. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.