Tumor breast cell exosomes and their role in cell adhesion during metastasis

• Grant number: 14/18747-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2015
• End date: August 01, 2019
• Field of knowledge: Biological Sciences - Biology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Heloisa Sobreiro Selistre de Araújo
• Grantee: Wanessa Fernanda Altei
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 13/00798-2 - The extracellular matrix in aging, exercise and in the tumor microenvironment, AP.TEM
• Associated scholarship(s): 16/22539-7 - The role of integrins on exosome binding to the extracellular matrix and its contribution to tumor progression, BE.EP.PD

Abstract

Metastasis occurs when tumor cells acquire characteristics of invasion and migration, being able to modify the microenvironment around them through interaction with extracellular matrix components, moving to a secondary site, developing a new focus of tumor. Exosomes are 40-100 nm extracellular vesicles, secreted by cells and involved in the information transport. It is believed that during metastasis, exosomes secretion by tumor cells have roles in both cell-cell adhesion tumor microenvironment and in distant regions of the primary tumor, thereby preparing the pre-metastatic niche. Thus, this research project aims to investigate the contribution of exosomes in metastatic process, through the investigation of their adhesion to the extracellular matrix in the primary tumor microenvironment, and the secondary focus of the tumor. These studies will be perfomed from the isolation and analysis of vesicles derived from breast cancer cell lines in tests of adhesion and invasion of the extracellular matrix. The importance of this project lies in the need for a deeper understanding of the process of metastasis, especially in intercellular communication required for the spreading of the tumor, aiming to find new and more effective therapies. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALTEI, WANESSA FERNANDA; PACHANE, BIANCA CRUZ; SOUZA, CRISTIANO; MARQUES, MARCIA MARIA CHIQUITELLI; SELISTRE-DE-ARAUJO, HELOISA. New insights into the discovery of drugs for triple-negative breast cancer metastasis. EXPERT OPINION ON DRUG DISCOVERY, v. 17, n. 4, p. 12-pg., 2022-02-20. (14/18747-8, 13/00798-2, 21/01983-4, 19/11437-7)

CRUSCA, JR., EDSON; MANSOR BASSO, LUIS GUILHERME; ALTEI, WANESSA FERNANDA; MARCHETTO, REINALDO. Biophysical characterization and antitumor activity of synthetic Pantinin peptides from scorpion's venom. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1860, n. 11, p. 2155-2165, NOV 2018. (14/18747-8, 14/00206-0)

ALTEI, WANESSA F.; PACHANE, BIANCA C.; DOS SANTOS, PATTY K.; RIBEIRO, LIGIA N. M.; SUNG, BONG HWAN; WEAVER, ALISSA M.; SELISTRE-DE-ARAUJO, HELOISA S.. Inhibition of alpha v beta 3 integrin impairs adhesion and uptake of tumor-derived small extracellular vesicles. CELL COMMUNICATION AND SIGNALING, v. 18, n. 1, p. 15-pg., 2020-09-25. (14/18747-8, 13/00798-2, 16/22539-7)

DANILUCCI, TAIS M.; SANTOS, PATTY K.; PACHANE, BIANCA C.; PISANI, GRAZIELE F. D.; LINO, RAFAEL L. B.; CASALI, BRUNA C.; ALTEI, WANESSA F.; SELISTRE-DE-ARAUJO, HELOISA S.. Recombinant RGD-disintegrin DisBa-01 blocks integrin alpha(v)beta(3) and impairs VEGF signaling in endothelial cells. CELL COMMUNICATION AND SIGNALING, v. 17, MAR 20 2019. (13/00798-2, 14/18747-8)