Evaluation of the role of TGF-² signaling pathway in prostatic induction: from the urogenital sinus to the ventral prostate primordium in mice and characterization of similar transitions in RWPE-1 cells

• Grant number: 15/03599-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2015
• End date: March 31, 2016
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Hernandes Faustino de Carvalho
• Grantee: Manoel Francisco Biancardi
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 09/16150-6 - Androgen regulation, sinalization and cellular interactions in prostate development, physiology and regression, AP.TEM

Abstract

Prostatic development is expression of different factors that are finely regulated temporally and spatially. Among these factors, TGF-²1 is related with important biological processes such as proliferation, cell differentiation, migration and apoptosis. However, little is known about the mechanisms modulated by TGF-²1 in initial events of prostatic development, particularly the budding from the urogenital sinus epithelium. Besides the functions related to the normal physiology of this gland, some studies have been shown that TGF-²1 has a determinant role on prostate cancer progression. Thus, based on the hypothesis that TGF-²1 has a determinant role on the prostate epithelial during development, the aim of this project is the characterization of the TGF-²1 signaling pathway during the initial prostate organogenesis. Towards this goal, we will employ in vivo experiments using the Swiss mice, and in vitro assays with ex-vivo cultures of the urogenital sinus (UGS) and with RWPE-1 cells. Moreover, we will apply morphological, immunocitochemical and in situ hybridization analyses. With this study, we want to understand the complex mechanisms that regulate the initial events of prostate organogenesis, especially those regulated by TGF-²1. Having a well established understanding of TGF-²1 signaling pathway and considering this factor an important therapeutic target, we pretend to use this model in future researches with drugs, searching for new treatments for prostate diseases. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BIANCARDI, MANOEL F.; DOS SANTOS, FERNANDA C. A.; DE CARVALHO, HERNANDES F.; SANCHES, BRUNO D. A.; TABOGA, SEBASTIAO R.. Female prostate: historical, developmental, and morphological perspectives. Cell Biology International, v. 41, n. 11, SI, p. 1174-1183, NOV 2017. (15/03599-6, 09/16789-7, 09/53990-2)

BIANCARDI, MANOEL F.; DOS SANTOS, FERNANDA C. A.; DE CARVALHO, HERNANDES F.; SANCHES, BRUNO D. A.; TABOGA, SEBASTIAO R.. Female prostate: historical, developmental, and morphological perspectives. Cell Biology International, v. 41, n. 11, p. 10-pg., 2017-11-01. (15/03599-6, 09/16789-7, 09/53990-2)