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MECHANISM OF ACTION OF TRIIODOTHYRONINE HORNONE (T3) IN THE TGFa GENE EXPRESSION IN BREAST ADENOCARCINOMA CELL LINES

Grant number: 15/09686-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2015
Effective date (End): June 30, 2016
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Celia Regina Nogueira
Grantee:Tabata Marinda da Silva
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated research grant:13/05629-4 - Genomic vs nongenomic actions of thyroid hormones: changes of paradigms, physiological implications and therapeutical perspectives, AP.TEM

Abstract

Several environmental risk factors, pathological conditions and physiological agents, as the thyroid hormones (TH), have been put foward for discussion for influencing the development of breast câncer (BC). Our group evinced that the TGF± expression is increased in the treatments with T3 in 24 hours, however this expression does not occur in cellular models that do not have the estrogen receptor or when cells are concomitantly treated with antiestrogen tamoxifen. In a previous study of our group, we demonstrated that the gene expression of HIF-1± and TGF± is increased in the presence T3 in supraphysiological dose, in the cell line of breast cancer in short times (10 '30' 1h and 4h). These findings lead us to new researches to elucidate the pathways of the T3 in physiological dose, through the use of specific drugs to block the intracellular signaling pathways, to approach the non-genomic actions of TH, such as PD98059 (inhibitor of ERK/MAPK) and Alpha-amanitin (RNA gene transcription-blocking lock Polymerase II). The above treatments will be performed in cell culture with breast adenocarcinoma cell lines (MCF-7), incubated with T3 in physiological dose 10-9M, in the presence and absence of these drugs, with subsequent analysis of gene expression (RT-PCR) of TGF± gene.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA, TABATA M.; MORETTO, FERNANDA C. F.; DE SIBIO, MARIA T.; GONCALVES, BIANCA M.; OLIVEIRA, MIRIANE; OLIMPIO, REGIANE M. C.; OLIVEIRA, DIEGO A. M.; COSTA, SARAH M. B.; DEPRA, IGOR C.; NAMBA, VICKELINE; NUNES, MARIA T.; NOGUEIRA, CELIA R. Triiodothyronine (T-3) upregulates the expression of proto-oncogene TGFA independent of MAPK/ERK pathway activation in the human breast adenocarcinoma cell line, MCF7. ARCHIVES OF ENDOCRINOLOGY METABOLISM, v. 63, n. 2, p. 142-147, MAR-APR 2019. Web of Science Citations: 1.
TABATA M. SILVA; FERNANDA C. F. MORETTO; MARIA T. DE SIBIO; BIANCA M. GONÇALVES; MIRIANE OLIVEIRA; REGIANE M. C. OLIMPIO; DIEGO A. M. OLIVEIRA; SARAH M. B. COSTA; IGOR C. DEPRÁ; VICKELINE NAMBA; MARIA T. NUNES; CÉLIA R. NOGUEIRA. Triiodothyronine (T3) upregulates the expression of proto-oncogene TGFA independent of MAPK/ERK pathway activation in the human breast adenocarcinoma cell line, MCF7. ARCHIVES OF ENDOCRINOLOGY METABOLISM, v. 63, n. 2, p. 142-147, Abr. 2019.

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