Threshold dose distribution and its causes and consequences in photodynamic therapy

• Grant number: 15/06579-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2015
• End date: February 28, 2017
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Vanderlei Salvador Bagnato
• Grantee: Clara Maria Gonçalves de Faria
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

There are many research groups in the field of photodynamic therapy (PDT). However, some challenges were not yet overcome, such as results variability. The aim of this project is to advance the state of art of the technique, investigating the causes for the variability. It will be used a threshold dose distribution model to evaluate cell resistance to PDT. From quantitative comparisons of the distribution curves acquired in different treatment conditions, it will be investigated the relationship between PDT parameters and cell resistance. Conditions include different photosensitizer concentration (Photogem and Clorin e6) and incubation time and light doses in cell and microorganisms culture. After the PDT applications, cell viability will be analyzed by MTT and fluorescence analysis. The Grupo de Óptica develop research in photodynamic therapy since 1999 and it is a reference in the field. Not only in research, but also at applications: it develops the largest Brazilian clinical projects: Skin Cancer and Cervical Cancer. The facilities available at the group are high-technology laboratories for cell culture, microorganisms and in vivo experiments, among others. There are several equipment available for measurements, such as Confocal Microscopes and Spectrometers (UV-VIS and FTIR). Therefore, we believe that this project will expand the understanding of PDT mechanisms, which allows the advance of the technique. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES DE FARIA, CLARA MARIA; INADA, NATALIA MAYUMI; KURACHI, CRISTINA; BAGNATO, VANDERLEI SALVADOR. Determination of the threshold dose distribution in photodynamic action from in vitro experiments. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 162, p. 168-175, SEP 2016. (15/06579-6)

GONSALVES DE FARIA, CLARA MARIA; INADA, NATALIA MAYUMI; VOLLET-FILHO, JOSE DIRCEU; BAGNATO, VANDERLEI SALVADOR. A threshold dose distribution approach for the study of PDT resistance development A threshold distribution approach for the study of PDT resistance. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 182, p. 85-91, MAY 2018. (09/54035-4, 13/07276-1, 15/06579-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FARIA, Clara Maria Gonçalves de. Threshold dose distributions and its causes and consequences in photodynamic therapy. 2017. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.