Tolerance induction and Candida albicans's biofilm characterization after antimicrobial photodymamic therapy application

• Grant number: 21/01191-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): May 01, 2021
• Effective date (End): May 02, 2024
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Ana Cláudia Pavarina
• Grantee: Túlio Morandin Ferrisse
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID
• Associated scholarship(s): 22/12828-2 - Interaction of Candida streptococcus multispecies biofilm in dental demineralization and oral candidosis, BE.EP.PD

Abstract

The fungus Candida albicans, in situations of imune imbalance in the host, can become an opportunist pathogen and cause infections. Due to the resistence that microorganism has developed to conventional drugs, antimicrobial photodynamic therapy (aPDT) has shown promising results as an alternative therapy. The aim of this study will be evaluate the induction ablity that aPDT has in developing tolerant biofilms of C. albicans in association with curcumin (CUR - 40 µg/L) and LED light (18 J/cm2). Three strains of C. albicans will be used in the presente study (ATCC 90028 and susceptible and resistant isolates) and being planted on Sabourand Dextrose Agar plates to obtain the biofilms. The biofilms will be submmited to successive applications of aPDT in a sub-lethal dose with the values of colony-forming units being evaluated in each application of aPDT. There will be four study groups evaluated, namely: aPDT (PS+Light), PS in absence of light, only application of light and control (absence of PS and light). Once tolerance was induced, the biofilms will be cultivated again for characterization and quantification of the polymeric extracellular matrix (total biomass analysis, protein quantification, water soluble polysaccharide quantification, alcohol soluble polysaccharide analysis, eDNA analysis and confocal and laser microscopy analysis) and for evaluation of expression of genes related with the adhesion of C. albicans to the substrate and genes responsibles for development of biofilms (ALS1, ALS3, EAP1 e PHR1). To confirm the normality and homocedasticity of the data the Shapiro-Wilk test and Levene's test will be perfom, respectively. For inference analyses, the analysis of variance (ANOVA) and Kruskall-Wallis will be conduct with a significant level of 5% (±=0.05). (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FRANCA, GABRIELA GOMES; DE ANNUNZIO, SARAH RAQUEL; KOPP, WILLIAN; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms. PATHOGENS, v. 12, n. 2, p. 19-pg., 2023-02-01. (19/08375-0, 18/23015-7, 18/18440-0, 13/07276-1, 21/01191-0, 20/07110-0)

SURUR, AMANDA KOBERSTAIN; MOMESSO, VINICIUS MEDEIROS; LOPES, PEDRO MONTEIRO; FERRISSE, TULIC MORANDIN; FONTANA, CARLA RAQUEL. Assessment of synergism between enzyme inhibition of Cu/Zn-SOD and antimicrobial photodynamic therapy in suspension and E. coli biofilm. Photodiagnosis and Photodynamic Therapy, v. 41, p. 8-pg., 2022-11-28. (21/01191-0, 19/26524-2, 13/07276-1)

FERRISSE, TULIO MORANDIN; DIAS, LUANA MENDONCA; DE OLIVEIRA, ANALU BARROS; JORDAO, CLAUDIA CAROLINA; DE OLIVEIRA MIMA, EWERTON GARCIA; PAVARINA, ANA CLAUDIA. Efficacy of curcumin-mediated antibacterial photodynamic therapy for oral antisepsis: A systematic review and network meta-analysis of randomized clinical trials. Photodiagnosis and Photodynamic Therapy, v. 39, p. 9-pg., 2022-05-30. (20/07110-0, 21/01191-0, 13/07276-1, 20/16227-8)

FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; SURUR, AMANDA KOBERSTAIN; BUZO, HELEN SORDI; BRIGHENTI, FERNANDA LOURENCAO; FONTANA, CARLA RAQUEL. Photodynamic therapy associated with nanomedicine strategies for treatment of human squamous cell carcinoma: A systematic review and meta-analysis. Nanomedicine-Nanotechnology Biology and Medicine, v. 40, p. 10-pg., 2022-02-01. (18/23015-7, 20/05169-7, 21/01191-0)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; DE ANNUNZIO, SARAH RAQUEL; FRANCA, MARIA GLEIZIANE ARAUJO; SILVA, MARIA GORETTI DE VASCONCELOS; CAVALHEIRO, ALBERTO JOSE; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. In Vitro Evaluation of Photodynamic Activity of Plant Extracts from Senna Species against Microorganisms of Medical and Dental Interest. PHARMACEUTICS, v. 15, n. 1, p. 16-pg., 2023-01-01. (13/07600-3, 22/12828-2, 14/50926-0, 20/07110-0, 19/08375-0, 18/09088-1, 18/18440-0, 18/23015-7, 21/01191-0)

FERRISSE, TULIO MORANDIN; DIAS, LUANA MENDONCA; DE OLIVEIRA, ANALU BARROS; JORDAO, CLAUDIA CAROLINA; DE OLIVEIRA MIMA, EWERTON GARCIA; PAVARINA, ANA CLAUDIA. Efficacy of Antimicrobial Photodynamic Therapy Mediated by Photosensitizers Conjugated with Inorganic Nanoparticles: Systematic Review and Meta-Analysis. PHARMACEUTICS, v. 14, n. 10, p. 18-pg., 2022-10-01. (19/27634-6, 20/07110-0, 20/16227-8, 21/01191-0, 13/07276-1)

FORNEL, DIOGENES GERMANO; FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; FONTANA, CARLA RAQUEL. Photodynamic Therapy Can Modulate the Nasopharyngeal Carcinoma Microenvironment Infected with the Epstein-Barr Virus: A Systematic Review and Meta-Analysis. BIOMEDICINES, v. 11, n. 5, p. 14-pg., 2023-05-02. (22/12828-2, 21/01191-0, 13/07276-1, 20/07110-0)

PALACON, MARIANA PARAVANI; BARBEIRO, CAMILA DE OLIVEIRA; FERNANDES, DARCY; BIANCARDI, MARIEL RUIVO; SILVEIRA, HEITOR ALBERGONI; FERRISSE, TULIO MORANDIN; LEON, JORGE ESQUICHE; KUJAN, OMAR; BUFALINO, ANDREIA. Macrophages CD163+and Factor XIIIa plus Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 6, p. 12-pg., 2023-03-01. (18/04954-2, 18/22236-0, 13/07276-1, 21/01191-0, 17/01798-7, 17/17288-8)

SILVESTRE, AMANDA LETICIA POLLI; DOS SANTOS, ALINE MARTINS; DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; BRIGHENTI, FERNANDA LOURENCAO; MENEGUIN, ANDREIA BAGLIOTTI; CHORILLI, MARLUS. Evaluation of photodynamic therapy on nanoparticles and films loaded-nanoparticles based on chitosan/alginate for curcumin delivery in oral biofilms. International Journal of Biological Macromolecules, v. 240, p. 13-pg., 2023-04-20. (21/01191-0, 18/23096-7, 20/07110-0)

SALES, LUCIANA SOLERA; MIRANDA, MARINA LINS; DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FONTANA, CARLA RAQUEL; MILWARD, MICHAEL; BRIGHENTI, FERNANDA LOURENCAO. Effect of the technique of photodynamic therapy against the main microorganisms responsible for periodontitis: A systematic review of in-vitro studies. ARCHIVES OF ORAL BIOLOGY, v. 138, p. 13-pg., 2022-03-28. (19/26066-4, 21/12931-5, 20/07110-0, 19/08375-0, 21/01191-0)

DIAS, LUANA MENDONCA; KLEIN, MARLISE INEZ; FERRISSE, TULIO MORANDIN; MEDEIROS, KARINE SOUSA; JORDAO, CLAUDIA CAROLINA; BELLINI, AMANDA; PAVARINA, ANA CLAUDIA. The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer. JOURNAL OF FUNGI, v. 9, n. 1, p. 22-pg., 2023-01-01. (18/14874-6, 19/27634-6, 21/01191-0, 13/07276-1)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; BASSO, FERNANDA GONCALVES; FONTANA, CARLA RAQUEL; APARECIDA GIRO, ELISA MARIA; BRIGHENTI, FERNANDA LOURENCAO. A systematic review and meta-analysis of the effect of photodynamic therapy for the treatment of oral mucositis. Photodiagnosis and Photodynamic Therapy, v. 34, JUN 2021. (18/18440-0, 20/07110-0, 21/01191-0, 19/08375-0, 18/23015-7)

GABBAI-ARMELIN, PAULO R.; SALES, LUCIANA S.; FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; DE OLIVEIRA, JULIANA R.; GIRO, ELISA M. A.; BRIGHENTI, FERNANDA L.. A systematic review and meta-analysis of the effect of thymol as an anti-inflammatory and wound healing agent A review of thymol effect on inflammation and wound healing. Phytotherapy Research, v. 36, n. 9, p. 29-pg., 2022-07-18. (20/07110-0, 19/08375-0, 21/01191-0, 19/26066-4)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FONTANA, CARLA RAQUEL; BASSO, FERNANDA GONCALVES; BRIGHETI, FERNANDA LOURENCAO. Photodynamic therapy for treating infected skin wounds: A systematic review and meta-analysis from randomized clinical trials. Photodiagnosis and Photodynamic Therapy, v. 40, p. 11-pg., 2022-12-01. (13/07276-1, 18/23015-7, 21/01191-0, 20/07110-0, 19/08375-0, 18/18440-0)

FERNANDES, DARCY; BARBEIRO, CAMILA DE OLIVEIRA; PALACON, MARIANA PARAVANI; BIANCARDI, MARIEL RUIVO; FERRISSE, TULIO MORANDIN; SILVEIRA, HEITOR ALBERGONI; CASTILHO, ROGERIO MORAES; DE ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE; BUFALINO, ANDREIA. High density of CD8 T cell and immune imbalance of T lymphocytes subsets are associated with proliferative verrucous leukoplakia. IMMUNOLOGY, v. 168, n. 1, p. 14-pg., 2022-09-03. (17/01798-7, 17/01438-0, 13/07276-1, 18/22236-0, 21/01191-0, 18/04954-2, 17/17288-8)

DIAS, LUANA MENDONCA; FERRISSE, TULIO MORANDIN; MEDEIROS, KARINE SOUSA; CILLI, EDUARDO MAFFUD; PAVARINA, ANA CLAUDIA. Use of Photodynamic Therapy Associated with Antimicrobial Peptides for Bacterial Control: A Systematic Review and Meta-Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 6, p. 14-pg., 2022-03-01. (21/06476-3, 21/01191-0, 20/16227-8, 13/07276-1)

GABBAI-ARMELIN, PAULO ROBERTO; DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; SALES, LUCIANA SOLERA; BARBOSA, ELIS RODRIGUES OLIVEIRA; MIRANDA, MARINA LINS; SALOMAO, KARINA BORGES; BRIGHENTI, FERNANDA LOURENCAO. COVID-19 (SARS-CoV-2) infection and thrombotic conditions: A systematic review and meta-analysis. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, v. 51, n. 6, APR 2021. (21/01191-0, 19/08375-0, 19/26066-4, 18/18440-0, 20/07110-0)