Highly parallelised SPR detection of biosamples on a large-scale-integrated microfluidic platform

• Grant number: 15/16311-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: January 01, 2016
• End date: December 23, 2016
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Marystela Ferreira
• Grantee: Celina Massumi Miyazaki
• Supervisor: Jens Ducree
• Host Institution: Centro de Ciências e Tecnologias para a Sustentabilidade (CCTS). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil
• Institution abroad: Dublin City University (DCU), Ireland
• Associated to the scholarship: 14/15093-7 - Study of nanostructured systems by surface plasmon resonance (SPR): applications in biosensing, BP.PD

Abstract

The centrifugal microfluidic platform seamlessly combines a network of liquid handling steps such as sample preparation, sedimentation, metering on a single, typically disc-shaped cartridge, thus enabling a full integration, automation and parallelization of bioanalytical protocols for decentralized bioanalytics with a user-friendly, widely autonomous instrument ("player"). We propose to develop a highly sensitive surface plasmon resonance (SPR) optical system integrated with such a "Lab-on-a-Disc" (LoaD) platform for the multiplexed detection of biomolecules. Due to its direct, label-free method SPR facilitates liquid handling and thus allows large-scale multiplexed detection of a range of analytes on the LoaD platform. The steps of microfluidic design engineering, coupling of SPR optical system, biofunctionalization and bioanalysis will be developed in this proposal. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIYAZAKI, CELINA M.; MISHRA, ROHIT; KINAHAN, DAVID J.; FERREIRA, MARYSTELA; DUCREE, JENS. Polyethylene imine/graphene oxide layer-by-layer surface functionalization for significantly improved limit of detection and binding kinetics of immunoassays on acrylate surfaces. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 158, p. 167-174, OCT 1 2017. (15/16311-0)

MIYAZAKI, CELINA M.; KINAHAN, DAVID J.; MISHRA, ROHIT; MANGWANYA, FAITH; KILCAWLEY, NIAMH; FERREIRA, MARYSTELA; DUCREE, JENS. Label-free, spatially multiplexed SPR detection of immunoassays on a highly integrated centrifugal Lab-on-a-Disc platform. BIOSENSORS & BIOELECTRONICS, v. 119, p. 86-93, NOV 15 2018. (15/16311-0)

DELGADO, SARAI M. TORRES; KINAHAN, DAVID J.; JULIUS, LOURDES ALBINA NIRUPA; MALLETTE, ADAM; ARDILA, DAVID SAENZ; MISHRA, ROHIT; MIYAZAKI, CELINA M.; KORVINK, JAN G.; DUCREE, JENS; MAGER, DARIO. Wirelessly powered and remotely controlled valve-array for highly multiplexed analytical assay automation on a centrifugal microfluidic platform. BIOSENSORS & BIOELECTRONICS, v. 109, p. 214-223, JUN 30 2018. (15/16311-0)

MIYAZAKI, CELINA M.; CARTHY, EADAOIN; KINAHAN, DAVID J.. Biosensing on the Centrifugal Microfluidic Lab-on-a-Disc Platform. PROCESSES, v. 8, n. 11, NOV 2020. (14/15093-7, 15/16311-0)