Hepatic metabolic analysis and oxidative stress in type 1 Diabetes

• Grant number: 15/04806-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2015
• Effective date (End): August 31, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Ana Angélica Henrique Fernandes
• Grantee: Lucas Rodolfo de Oliveira Rosa
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The objective of this project is to evaluate the effect of N-acetylcysteine (NAC) on metabolic parameters and oxidative stress in the hepatic tissue of rats with type 1 diabetes mellitus. 32 Wistar rats (± 250g BW)will be distributed in experimental groups (n=8): G1 (normal, control); G2 (normal, treated with NAC); G3 (untreated diabetics); G4 (diabetics, treated with NAC). Experimental Diabetes mellitus is induced by intraperitoneal administration of streptozotocin (STZ), at a single dose, on concentration of 60mg/kg-1 BW. With the establishment of the diabetic state, animals from groups G2 and G4 will receive NAC in the concentration 30mg/day, by intra-gastric route (gavage), for 30 days. At end of experimental period the animals will be euthanized by cervical fracture and decapitation. The blood will be collected and serum separated by centrifugation and used for determinations of glucose, triacylglycerois, total cholesterol, HDL-cholestrol. The samples (200mg) of hepatic, are stored in a "freezer" to -80 ° C for the determination of the concentration of total protein, triacylglycerois, lipid hydroperoxide, glutathione peroxidase, superoxide dismutase and catalase, activity of marker enzymes of energy metabolism, lactate dehydrogenase, citrate synthase and pyruvate dehydrogenase. The statistical design is entirely randomized with four treatments and eight repetitions, according to analysis of variance (ANOVA). The significance level is 5% probability and the F statistics are significant at p <0.05. Tukey's test will be used for comparison between average.